Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase MEDEA (MEA), partial

Code CSB-YP524055DOA
MSDS
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Source Yeast
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Code CSB-EP524055DOA
MSDS
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Source E.coli
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Code CSB-EP524055DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP524055DOA
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Source Baculovirus
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Code CSB-MP524055DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MEA
Uniprot No.
Alternative Names
MEA; EMB173; FIS1; MEDEA; SDG5; SET5; At1g02580; T14P4.11Histone-lysine N-methyltransferase MEDEA; EC 2.1.1.356; Maternal embryogenesis control protein; Protein EMBRYO DEFECTIVE 173; Protein FERTILIZATION-INDEPENDENT SEED 1; Protein SET DOMAIN GROUP 5
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Polycomb group (PcG) protein. Catalytic subunit of some PcG multiprotein complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. Required to prevent the proliferation of the central cell of the female gametophyte by repressing target genes before fertilization. After fertilization, it probably also regulates the embryo and endosperm proliferation and anteroposterior organization during seed development. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. Interacts with the promoter and repress the transcription of genes such as PHE1 and PHE2, that are paternally active and maternally silenced genes.
Gene References into Functions
  1. TAF13 functions together with MEDEA and SWINGER, both members of a plant variant of Polycomb Repressive Complex 2 (PRC2)in transcriptional regulation during seed development. [MEDEA] PMID: 23506837
  2. functional requirement of the FIS-PRC2 is to balance parental genome contributions and to reduce genetic conflict. PMID: 23326241
  3. imprinted MEA expression mediated by this ICR is independent of DME and MET1, consistent with the lack of any significant DNA methylation in this region PMID: 22855791
  4. Parent-of-origin-specific expression of the Polycomb group (PcG) gene MEDEA is causally responsible for seed developmental aberrations in Arabidopsis seeds with increased paternal genome contributions. PMID: 19779546
  5. MEA imprinting is regulated by histone methylation. PMID: 16527743
  6. MEA-FIE PcG complex implies distinct functions that are dynamically regulated during reproduction. PMID: 16651654
  7. MEA and SWN perform partially redundant functions in controlling the initiation of endosperm development before fertilization in Arabidopsis. PMID: 16924116
  8. The evolution of MEA suggests a late origin of genomic imprinting within the Brassicaceae, whereas imprinting is thought to have originated early within the mammalian lineage PMID: 17637669
  9. Study presents data and analyses of sequence diversity of MEA in self-fertilizing and outcrossing Arabidopsis and its relatives, to help clarify the form of selection acting on this gene. PMID: 19126870

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Subcellular Location
Nucleus. Note=Excluded from the nucleolus.
Protein Families
Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, EZ subfamily
Tissue Specificity
Expressed in unpollinated siliques that contain maturing gametophytes. Not expressed at early stages of floral development during early megagametogenesis.
Database Links

KEGG: ath:AT1G02580

STRING: 3702.AT1G02580.1

UniGene: At.10786

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