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Recombinant Arabidopsis thaliana Inactive leucine-rich repeat receptor-like protein kinase CORYNE (CRN), partial

Recombinant Arabidopsis thaliana Inactive leucine-rich repeat receptor-like protein kinase CORYNE (CRN), partial

Code	CSB-YP888868DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP888868DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP888868DOA-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP888868DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP888868DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

CRN

Uniprot No.

Q9LYU7

Alternative Names

CRN; SOL2; At5g13290; T31B5.110; Inactive leucine-rich repeat receptor-like protein kinase CORYNE; Protein SUPPRESSOR OF OVEREXPRESSION OF LLP1 2

Species

Arabidopsis thaliana (Mouse-ear cress)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Involved in the perception of CLV3 and CLV3-like (CLE) peptides, that acts as a extracellular signals regulating meristems maintenance. Modulates root, shoot and flower apical meristems maintenance and floral organ development regulation, probably via CLAVATA (CLV)-like pathways involving at least CLV3 and CLE19. In complex with CLV2, perceives secreted CLV3-like effector proteins from plant-parasitic cyst nematodes as ligand mimics of the plant CLE signaling pathway. This recognition is required for proper feeding structure (syncytium) development and ultimately successful nematode infection. CLE14 perception by CLV2/CRN complex triggers root meristem differentiation. Required for the sensing of the root CLE peptides (e.g. CLE8, CLE9/CLE10, CLE11, CLE13, CLE14, CLE16, CLE17, CLE18, CLE20, CLE21, CLE25, CLE26, CLE40, CLE41/CLE44 and CLE45), which involves also CLV2 and leads to root growth regulation, mostly in the phloem and protophloem. Promotes the accumulation of BAM3, especially at later stages of protophloem development.

Gene References into Functions

study reports that the receptor-like protein CLAVATA 2 (CLV2) and the pseudokinase CORYNE (CRN) are necessary to fully sense root-active CLE peptides; CRN stabilizes BAM3 expression and thus is required for BAM3-mediated CLE45 signaling. PMID: 28607033
Our results suggest a model in which CLV1/BAM1 and CLV2/CRN complexes act in separate, parallel pathways in shoot meristems, while the CLV1, CLV2, and CRN receptors function together in a linear pathway during fruit development. PMID: 21705761
A 12-mer random-peptide library was used to determine the specific amino acid sequences binding with the recombinant CRN molecule. PMID: 21214859
CRN contains features of a pseudokinase but lacks kinase activity PMID: 21398569
The results therefore imply that the CLE14/CLE20 peptides may act through the CLV2-CRN receptor kinase, and that their availabilities and/or abundances may be affected by cytokinin activity in planta. PMID: 20697738
This study suggested that the CLV3 signal is perceived by a tetrameric CLV2/CRN complex and a CLV1 homodimer that localize to the PM and can interact via CRN. PMID: 19933383
The novel receptor kinase CORYNE (CRN) and CLV2 act together, and in parallel with CLV1, to perceive the CLV3 signal. [CORYNE] [CRN] PMID: 18381924
SOL2, together with CLV2, plays an important role in the regulation of root meristem development through the CLE signaling pathway. [SOL2] PMID: 18854335

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Tissue Specificity

Present in roots, stems, leaves, inflorescence, flowers and siliques. Mostly expressed in shoot tips and, to a lesser extent, in young organs and roots. Also expressed in the inner tissues of the proximal root meristem. Expressed in the vascular cylinder

Database Links

KEGG: ath:AT5G13290

STRING: 3702.AT5G13290.2

UniGene: At.124

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