Recombinant Arabidopsis thaliana Inositol-3-phosphate synthase isozyme 1 (At4g39800), partial

Code CSB-YP337218DOA
MSDS
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Source Yeast
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Code CSB-EP337218DOA
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Source E.coli
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Code CSB-EP337218DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP337218DOA
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Source Baculovirus
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Code CSB-MP337218DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
IPS1
Uniprot No.
Alternative Names
IPS1; MIPS1; At4g39800; T19P19.190; Inositol-3-phosphate synthase isozyme 1; AtIPS1; MIP synthase 1; EC 5.5.1.4; Myo-inositol 1-phosphate synthase 1; AtMIPS 1; MI-1-P synthase 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the majority of myo-inositol synthesis required for plant growth and development. Acts as a repressor of programmed cell death and protects plant cells against cell death under high light intensity or long days. Controls its own transcription by inhibiting ATXR6 activity. Reduces the deposition of inhibitory histone marks on its own promoter.
Gene References into Functions
  1. works with MIPS2 AND MIPS3 to impact the overall synthesis of myo-inositol and overall inositol homeostasis PMID: 29777485
  2. chlorophyll accumulation Is required for the onset of programmed cell death (PCD) in myoinositol phosphate synthase1 (mips1) mutant. PMID: 26747283
  3. MIPS1 binds directly to its promoter to stimulate its own expression by locally inhibiting the spreading of ATXR5/6-dependent heterochromatin marks coming from a transposable element. PMID: 23341037
  4. MIPS1 has a significant impact on myo-inositol levels that is critical for maintaining levels of ascorbic acid, phosphatidylinositol, and ceramides that regulate growth, development, and cell death. PMID: 20215587
  5. Though MIPS localizes to the cytosol primarily in the endosperm during seed development, myo-inositol-6-phosphate, the final product of inositol phosphate metabolism, was present mainly in the embryo. PMID: 18603618

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Myo-inositol 1-phosphate synthase family
Tissue Specificity
Expressed in siliques, leaves, roots, seed endosperm, but not in embryos. Highest expression in leaves, but restricted to vascular tissue in older leaves.
Database Links

KEGG: ath:AT4G39800

STRING: 3702.AT4G39800.1

UniGene: At.24599

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