Recombinant Arabidopsis thaliana Leucine-rich repeat receptor-like protein CLAVATA2 (CLV2), partial

Code CSB-YP525705DOA
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Source Yeast
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Code CSB-EP525705DOA
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Source E.coli
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Code CSB-EP525705DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP525705DOA
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Source Baculovirus
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Code CSB-MP525705DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CLV2
Uniprot No.
Alternative Names
CLV2; RLP10; At1g65380; T8F5.16Receptor-like protein CLAVATA2; Receptor-like protein 10; AtRLP10
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the perception of CLV3 and CLV3-like (CLE) peptides, that act as extracellular signals regulating meristems maintenance. Required for the sensing of the root CLE peptides (e.g. CLE8, CLE9/CLE10, CLE11, CLE13, CLE14, CLE16, CLE17, CLE18, CLE20, CLE21, CLE25, CLE26, CLE40, CLE41/CLE44 and CLE45), which involves also CRN and leads to root growth regulation, mostly in the phloem and protophloem. Involved in controlling the stem cell population size in shoot and root apical meristems, and during organ development. Promotes the formation of CLV1 multimers. In complex with CRN, perceives secreted CLV3-like effector proteins from plant-parasitic cyst nematodes as ligand mimics of the plant CLE signaling pathway. This recognition is required for proper feeding structure (syncytium) development and ultimately successful nematode infection. CLE14 perception by CLV2/CRN complex triggers root meristem differentiation.
Gene References into Functions
  1. study reports that the receptor-like protein CLAVATA 2 (CLV2) and the pseudokinase CORYNE (CRN) are necessary to fully sense root-active CLE peptides PMID: 28607033
  2. CLV1 and CLV2, two receptors involved in CLV3 perception during plant development, contribute to bacterial wilt through a signalling pathway involving the miR169/NF-YA module. PMID: 26990325
  3. Gene NANA regulates cell proliferation in Arabidopsis thaliana shoot apical meristem without interaction with CLV2. PMID: 25752149
  4. Over-expression of CLV2 in wild-type plants unexpectedly resulted in a multi-carpel phenotype, mimicking the clv2 mutant, which suggests a co-suppression effect on the endogenous CLV2. PMID: 20961653
  5. Data report detection of distinct CLV2-CRN heteromultimeric and CLV1-BAM multimeric complexes in transient expression in tobacco and in Arabidopsis meristems. PMID: 20626648
  6. tested the hypothesis by replacing these cysteines with alanines and showed that depletions of one or both of the cysteine pairs do not hamper the function of CLV2 in SAM maintenance PMID: 20738721
  7. Studies evaluating the functional specificity of of CLV2 and its related proteins are reported. PMID: 19897604

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Subcellular Location
Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Protein Families
RLP family
Tissue Specificity
Mostly expressed in apices (e.g. shoot apical meristem and flower buds), and, to a lower extent, in flowers, leaves, seedlings and siliques. Also expressed in the inner tissues of the proximal root meristem. Expressed throughout the vascular cylinder of r
Database Links

KEGG: ath:AT1G65380

STRING: 3702.AT1G65380.1

UniGene: At.17109

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