Recombinant Arabidopsis thaliana Nudix hydrolase 7 (NUDT7)

Code CSB-YP890440DOA
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP890440DOA
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP890440DOA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP890440DOA
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP890440DOA
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
NUDT7
Uniprot No.
Alternative Names
NUDT7; GFG1; NUDX7; At4g12720; T20K18.70; Nudix hydrolase 7; AtNUDT7; EC 3.6.1.-; ADP-ribose pyrophosphatase; EC 3.6.1.13; NADH pyrophosphatase; EC 3.6.1.22; Protein GROWTH FACTOR GENE 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-282
Target Protein Sequence
MGTRAQQIPL LEGETDNYDG VTVTMVEPMD SEVFTESLRA SLSHWREEGK KGIWIKLPLG LANLVEAAVS EGFRYHHAEP EYLMLVSWIS ETPDTIPANA SHVVGAGALV INKNTKEVLV VQERSGFFKD KNVWKLPTGV INEGEDIWTG VAREVEEETG IIADFVEVLA FRQSHKAILK KKTDMFFLCV LSPRSYDITE QKSEILQAKW MPIQEYVDQP WNKKNEMFKF MANICQKKCE EEYLGFAIVP TTTSSGKESF IYCNADHAKR LKVSRDQASA SL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can use both NADH and ADP-ribose as substrates, but not 8-oxo-dGTP, cyclic ADP-ribose, GDP-manose, UDP-glucose, ATP, or GTP. Exerts negative control of EDS1 signaling.
Gene References into Functions
  1. The active and inactive AtNUDX6 proteins induced the expression of thioredoxin-h5 while the active and inactive AtNUDX7 proteins suppressed the accumulation of SA and subsequent gene expression, indicating that AtNUDX6 and 7 proteins themselves play distinct roles in stress responses. [AtNUDX7] PMID: 27095738
  2. provide evidence that AtERF1 is important for regulating AtNudt7 during oxidative signaling. PMID: 25451743
  3. AtNUDT7 is important for NAD:NADH homeostasis in seeds. PMID: 24504697
  4. NUDT7 forms complexes in vitro and in vivo with regulatory proteins involved in signal transduction. PMID: 22068106
  5. AtNUDT7 protein levels are induced in response to biotic and abiotic stresses. AtNUDT7 plays an important role in maintaining redox homeostasis, particularly for maintaining NADH:NAD+ balance for normal growth and development. PMID: 20704736
  6. Under oxidative stress, AtNUDX7 serves to maintain NAD(+) levels by supplying ATP via nucleotide recycling from free ADP-Rib molecules and thus regulates the defense mechanisms against oxidative DNA damage via modulation of the PAR reaction. [AtNUDX7] PMID: 19656905
  7. GFG1 is a key gene linking cellular metabolism and oxidative signaling. PMID: 16328543
  8. FMO1 and NUDT7 are important for signaling through an EDS1-dependent but SA-independent branch of plant defense and NUDT7 exerts negative control of EDS1 signaling. [NUDT7] PMID: 16531493
  9. functions to modulate the defense response to prevent excessive stimulation, ADP-ribose and NADH are preferred substrates PMID: 17660350
  10. structure-activity studies: mutation of specific residues in AtNUDT7 alters function; mutation of residues F73 & V168 disallows dimer formation & enzymatic activity; mutation of residues V69, F73, & V168 disrupts binding to regulatory 14-3-3 protein PMID: 19448856

Show More

Hide All

Subcellular Location
Nucleus. Cytoplasm. Cell membrane. Note=Localized at the plasma membrane when in complex with GG2, but present in the cytoplasm when associated with GG1. Detected in the cytoplasm and nucleus when interacting with RACK1A.
Protein Families
Nudix hydrolase family
Tissue Specificity
Expressed in stems, leaves, roots, flowers and siliques.
Database Links

KEGG: ath:AT4G12720

UniGene: At.23449

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1