Recombinant Arabidopsis thaliana Peroxisome biogenesis protein 5 (PEX5), partial

Code CSB-YP872288DOA
MSDS
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Source Yeast
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Code CSB-EP872288DOA
MSDS
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Source E.coli
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Code CSB-EP872288DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP872288DOA
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Source Baculovirus
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Code CSB-MP872288DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PEX5
Uniprot No.
Alternative Names
PEX5; At5g56290; MXK23.3; Peroxisome biogenesis protein 5; Peroxin-5; AtPEX5; Peroxisomal targeting signal type 1 receptor; Pex5p
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Import receptor for peroxisomal-targeting signal one (PTS1). A receptor-cargo complex composed of PEX5, PEX7, a PTS1-containing protein and a PTS2-containing protein is targeted to peroxisomes during import. Necessary for the developmental elimination of obsolete peroxisome matrix proteins.
Gene References into Functions
  1. PEX5 binding to the PEX14N-terminal domain (PEX14N) has no effect on the affinity of PEX5 for a PTS1 containing peptides. PMID: 24879895
  2. PEX7 facilitates PEX5 accumulation and import of PTS1 cargo into peroxisomes PMID: 20130089
  3. Pex5p and Pex7p play different roles in the maintenance of glyoxysomal and leaf peroxisomal functions in plants PMID: 15637057
  4. Pex5p recognizes Cat1 in a manner distinct from typical PTS1. PMID: 18308759
  5. The Arabidopsis pex5-1 mutant is specifically defective in PTS2 (but not PTS1) protein import into peroxisomes, probably because of reduced interaction with the PEX7 PTS2 receptor. PMID: 15548601
  6. A genetic screen for peroxisome defective mutants revealed a pex5 mutant that is sucrose dependent during germination and resistant to the inhibitory effects of IBA on root elongation. PMID: 11063705

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Subcellular Location
Cytoplasm. Peroxisome membrane; Peripheral membrane protein.
Protein Families
Peroxisomal targeting signal receptor family
Tissue Specificity
Expressed in flowers, siliques, leaves and roots.
Database Links

KEGG: ath:AT5G56290

STRING: 3702.AT5G56290.1

UniGene: At.10824

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