Recombinant Arabidopsis thaliana Polyamine oxidase 1 (PAO1)

Code CSB-YP884307DOA
MSDS
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Source Yeast
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Code CSB-EP884307DOA
MSDS
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Source E.coli
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Code CSB-EP884307DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP884307DOA
MSDS
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Source Baculovirus
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Code CSB-MP884307DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PAO1
Uniprot No.
Alternative Names
PAO1; PAO; At5g13700; MSH12.17; Polyamine oxidase 1; AtPAO1; EC 1.5.3.16; EC 1.5.3.17; N(1)-acetylpolyamine oxidase; Spermine oxidase
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-472
Target Protein Sequence
MSTASVIIIG AGISGISAAK VLVENGVEDV LILEATDRIG GRIHKQNFGD VPVELGAGWI AGVGGKESNP VWELASRFNL RTCFSDYTNA RFNIYDRSGK IFPTGIASDS YKKAVDSAIL KLKSLEAQCS GQVAEEAPSS PKTPIELAID FILHDFEMAE VEPISTYVDF GEREFLVADE RGYECLLYKM AEEFLVTSHG NILDYRLKLN QVVREVQQSR NGVVVKTEDG SVYEANYVIV SASIGVLQSD LLSFQPLLPR WKTEAIQKCD VMVYTKIFLK FPQCFWPCGP GQEFFIYAHE QRGYFTFWQH MENAYPGSNI LVVTLTNEQS KRVEAQSDQE TMKEAMSVLR DMFGATIPYA TDILVPRWWN NRFQRGSYSN YPMISDNQLL QNIKAPVGRI FFTGEHTSEK FSGYVHGGYL AGIDTSKSLL EEMKQSLLLQ PLLAFTESLT LTHQKPNNSQ IYTNVKFISG TS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Flavoenzyme involved in polyamine back-conversion. Catalyzes the oxidation of the secondary amino group of polyamines, such as spermine and its acetyl derivatives. Substrate preference is thermospermine > norspermine > spermine > N(1)-acetylspermine. No activity detected when putrescine, spermidine or N(1)-acetylspermidine are used as substrates. Plays an important role in the regulation of polyamine intracellular concentration (Probable). Involved in the production of hydrogen peroxide in response to salt and cold stresses.
Gene References into Functions
  1. AtPAO1 is the first plant Polyamine oxidase reported to be involved in a polyamine back-conversion pathway. PMID: 16778015
Subcellular Location
Cytoplasm.
Protein Families
Flavin monoamine oxidase family
Tissue Specificity
Expressed at very low levels in leaves, stems and inflorescences.
Database Links

KEGG: ath:AT5G13700

STRING: 3702.AT5G13700.1

UniGene: At.49017

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