Recombinant Arabidopsis thaliana Potassium channel SKOR (SKOR), partial

Code CSB-YP864960DOA
MSDS
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Source Yeast
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Code CSB-EP864960DOA
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Source E.coli
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Code CSB-EP864960DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP864960DOA
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Source Baculovirus
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Code CSB-MP864960DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SKOR
Uniprot No.
Alternative Names
SKOR; At3g02850; F13E7.21; Potassium channel SKOR; Stelar K(+) outward rectifying channel
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Highly selective outward-rectifying potassium channel. Involved in potassium release into the xylem sap toward the shoots. Assuming opened or closed conformations in response to the voltage difference across the membrane, the channel is activated by depolarization. The voltage-dependence of the channel is abolished by internal or external acidification. May interact with the cytoskeleton or with regulatory proteins.
Gene References into Functions
  1. Data show that nutritional conditions as a critical factor for the joint activity of Arabidopsis proteins SKOR and NPF7.3/NRT1.5 for shoot potassium (K) homeostasis. PMID: 26508776
  2. Study found that, in particular, the N-terminal part of the S5 played a critical role in KAT1 and SKOR gating. PMID: 20007672
  3. A minimal cysteine motif required to activate the SKOR K+ channel of Arabidopsis by the reactive oxygen species H2O2. PMID: 20605786
  4. Phosphatidylinositol phosphates were identified as essential regulators for the voltage-dependent and -independent activation of A. thaliana potassium channels expressed in X. laevis oocytes. PMID: 15842627
  5. The intracellular potassium sensing mechanism couples SKOR activity to potassium nutrition status in the 'source cells', establishing a supply-based unloading system for the regulation of potassium distribution. PMID: 16623888
  6. Mapping the SKOR sequence to the crystal structure of the voltage-dependent K+ channel suggests interaction between the S6 gating domain and the base of the pore helix, a prediction supported by mutations at this site. PMID: 16623889
  7. Single mutations in SKOR convert it from an outward K+ channel into an inward K+ channel. PMID: 18287042

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Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Potassium channel family, Plant (TC 1.A.1.4) subfamily
Tissue Specificity
Expressed in root pericycle and xylem parenchyma, and in flower at a lower level.
Database Links

KEGG: ath:AT3G02850

STRING: 3702.AT3G02850.1

UniGene: At.41071

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