Recombinant Arabidopsis thaliana Probable auxin efflux carrier component 6 (PIN6), partial

Code CSB-YP874485DOA
MSDS
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Source Yeast
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Code CSB-EP874485DOA
MSDS
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Source E.coli
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Code CSB-EP874485DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP874485DOA
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Source Baculovirus
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Code CSB-MP874485DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PIN6
Uniprot No.
Alternative Names
PIN6; At1g77110; F22K20.18; Auxin efflux carrier component 6; AtPIN6
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of the intracellular auxin-transport pathway. Regulates auxin transport and auxin homeostasis. Directly involved in the regulation of nectar production. Involved in unfolded protein response (UPR) activation. Involved in the control of vein patterning. Redundantly with PIN8, inhibits the vein-formation-promoting functions of PIN5. PIN5, PIN6, and PIN8 control vein network geometry, but they are expressed in mutually exclusive domains of leaf vascular cells.
Gene References into Functions
  1. PIN6 role in the control of auxin transport and homeostasis during auxin-mediated development including the lateral and adventitious root organogenesis PMID: 27240710
  2. preferentially expressed in the vasculature of the primary root, cotyledons, cauline leaves, floral stem, sepals, main transmitting tract of reproductive silique PMID: 24487186
  3. Data indicate that PIN6 regulates auxin homeostasis during plant development. PMID: 23922907
  4. PIN6 is required for proper auxin response and nectary function in Arabidopsis. PMID: 23551385
  5. Expression of PIN6 is positively correlated with total nectar production in Arabidopsis and is required for the proper development of short stamens. PMID: 23551385

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
Auxin efflux carrier (TC 2.A.69.1) family
Tissue Specificity
Expressed in the vasculature of the primary root, cotyledons, floral stem, sepals and the main transmitting tract of the reproductive silique. Expressed in embryos, shoot meristem, root tip and lateral root meristems. Expressed in the nectaries and the fl
Database Links

KEGG: ath:AT1G77110

STRING: 3702.AT1G77110.1

UniGene: At.11574

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