Recombinant Arabidopsis thaliana Probable lysine-specific demethylase JMJ14 (JMJ14), partial

Code CSB-YP818105DOA
MSDS
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Source Yeast
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Code CSB-EP818105DOA
MSDS
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Source E.coli
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Code CSB-EP818105DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP818105DOA
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Source Baculovirus
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Code CSB-MP818105DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
JMJ14
Uniprot No.
Alternative Names
JMJ14; JMJ4; PKDM7B; At4g20400; F9F13.50Probable lysine-specific demethylase JMJ14; EC 1.14.11.-; Jumonji domain-containing protein 14; Jumonji domain-containing protein 4; Lysine-specific histone demethylase JMJ14; Protein JUMONJI 14
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional repressor. Histone demethylase that demethylates 'Lys-4' (H3K4me) of histone H3 with a higher activity for H3K4me3 and H3K4me2 than H3K4me1. No activity on H3K9me3/2, H3K36me3/2 and H3K27me3/2. Represses FT and TSF expression to inhibit the floral transition. Binds around the transcription start site of the FT locus. Involved in the DRM2-mediated maintenance of DNA methylation, but not required for the de novo DNA methylation. Required for demethylating histone H3K4me3 at the target of RNA silencing. Together with NAC051/NAC052 and NAC050, regulates gene expression and flowering time, probably by the promotion of RNA-mediated gene silencing.
Gene References into Functions
  1. Plants defective in JMJ14 exhibit reduced Histone3 Lysine9 Lysine14 acetylation (H3K9K14Ac) levels, reduced polymerase II (Pol II) occupancy, and reduced transgene transcription. PMID: 23001035
  2. JMJ14 is specifically involved in the maintenance phase of DRM2-mediated RNA-directed DNA methylation. [Jmj14] PMID: 21052090
  3. JMJ14 acts downstream from the Argonaute effector complex to demethylate histone H3K4 at the target of RNA silencing. [JMJ14] PMID: 20478993
  4. AtJmj4 and ELF6 are H3K4 demethylases directly repressing FT chromatin and preventing precocious flowering in Arabidopsis. [AtJmj4] PMID: 19946624
  5. PKDM7B is involved in H3K4 demethylation in FT and TSF chromatin, and represses expression of these genes to inhibit the floral transition. PMID: 20202164
  6. JMJ14 (At4g20400), is an active histone H3K4 demethylase and is involved in flowering time regulation. PMID: 20177424

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Subcellular Location
Nucleus, nucleoplasm. Nucleus. Note=Not detected in the nucleolus and the chromocenters.
Protein Families
JARID1 histone demethylase family
Tissue Specificity
Expressed in shoot apex, primary root tip, trichomes of young leaves, leaf vascular tissues, anther filaments and styles. Detected in inflorescences, leaves, stems, roots and siliques. Mostly expressed in floral organs, and, at low levels, in other organs
Database Links

KEGG: ath:AT4G20400

STRING: 3702.AT4G20400.1

UniGene: At.43482

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