Recombinant Arabidopsis thaliana Probable polyamine oxidase 5 (PAO5), partial

Code CSB-YP879843DOA
MSDS
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Source Yeast
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Code CSB-EP879843DOA
MSDS
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Source E.coli
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Code CSB-EP879843DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP879843DOA
MSDS
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Source Baculovirus
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Code CSB-MP879843DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PAO5
Uniprot No.
Alternative Names
PAO5; At4g29720; T16L4.230; Probable polyamine oxidase 5; AtPAO5; EC 1.5.3.-
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Flavoenzyme involved in polyamine back-conversion. Catalyzes the oxidation of the secondary amino group of polyamines, such as spermine and its acetyl derivatives. Substrate preference is spermine > N(1)-acetylspermine > thermospermine > norspermine. Plays an important role in the regulation of polyamine intracellular concentration. Involved in xylem differentiation by controlling thermospermine homeostasis, and participating in the tightly controlled interplay between auxin and cytokinin that is necessary for proper xylem differentiation. Involved in the production of hydrogen peroxide in response to salt and cold stresses.
Gene References into Functions
  1. Two independent loss-of-function mutants (atpao5-2 and atpao5-3) were found to exhibit constitutively higher thermospermine levels, with associated increased salt tolerance. PMID: 26791972
  2. AtPAO5, being involved in the control of Therm-Spm homeostasis, participates in the tightly controlled interplay between auxin and cytokinins that is necessary for proper xylem differentiation. PMID: 28199662
  3. results suggest that rice PAO1 is the functional ortholog of Arabidopsis PAO5 PMID: 25763711
  4. AtPAO5 is a cytosolic protein undergoing proteasomal control. Positive regulation of AtPAO5 expression by polyamines is at the transcriptional and post-transcriptional level. PMID: 24550437
  5. flavin amine oxidase (FAO1) affects shoot regeneration by regulating cellular polyamines. [FAO1] PMID: 17122409

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Subcellular Location
Cytoplasm.
Protein Families
Flavin monoamine oxidase family
Tissue Specificity
Expressed in root vasculature, leaves and stems.
Database Links

KEGG: ath:AT4G29720

STRING: 3702.AT4G29720.1

UniGene: At.31894

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