Recombinant Arabidopsis thaliana Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 3 (ARC3), partial

Code CSB-YP739097DOA
MSDS
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Source Yeast
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Code CSB-EP739097DOA
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Source E.coli
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Code CSB-EP739097DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP739097DOA
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Source Baculovirus
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Code CSB-MP739097DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARC3
Uniprot No.
Alternative Names
ARC3; At1g75010; F25A4.3; F9E10.14Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Z-ring accessory protein involved in the initiation of plastid division and division site placement (might functionally replace bacterial MinC). May control the rate of chloroplast expansion. Seems to influence stromule (stroma-filled tubular extensions of the plastid envelope membrane) length and frequency.
Gene References into Functions
  1. Two new alleles of arc3 in Arabidopsis thaliana, arc3-4 and arc3-5, were isolated in the Columbia-0 ecotype. PMID: 23070302
  2. ARC3 is a stromal Z-ring accessory protein that interacts specifically with AtFtsZ1, and is essential for plastid division. [ARC3] PMID: 17304239
  3. FtsZ1-FtsZ2-ARC3-ARC6 complex represents an unpolymerized IEM-associated pool of FtsZ that contributes to the dynamic regulation of Z-ring assembly and remodelling at the plastid division site in vivo. [ARC3] PMID: 18284374
  4. In hypocotyls, the effects of the arc3 and arc6 mutations on stromule length and frequency are independent of changes in plastid division. [ARC3] PMID: 18764889
  5. Arc3 encodes a chloroplast division factor and arc mutants have large but fewer chloroplasts which have an effect on both the composition and structure of the photosynthetic apparatus. [ARC3] PMID: 16170638

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Subcellular Location
Plastid, chloroplast outer membrane; Peripheral membrane protein; Cytoplasmic side. Plastid, chloroplast stroma. Note=Located at the site of chloroplast division on the outer surface in a ring-shaped structure at the early and middle stages of the process according to PubMed:15356321. Forms ring-like structures, short filaments and discrete foci in the chloroplast stroma according to PubMed:17304239.
Database Links

KEGG: ath:AT1G75010

STRING: 3702.AT1G75010.1

UniGene: At.34822

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