Recombinant Arabidopsis thaliana Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 6, chloroplastic (ARC6), partial

Code CSB-YP887765DOA1
MSDS
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Source Yeast
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Code CSB-EP887765DOA1
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Source E.coli
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Code CSB-EP887765DOA1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887765DOA1
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Source Baculovirus
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Code CSB-MP887765DOA1
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARC6
Uniprot No.
Alternative Names
ARC6; At5g42480; MDH9.18; Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 6, chloroplastic
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the plastid division machinery. Involved in the initiation of proplastid and plastid division (including chloroplasts, statoliths and leukoplasts). Promotes the assembly and/or stabilization of the plastid-dividing FtsZ ring, functioning as an antagonistic regulator of FtsZ dynamics against CDP1. Relays plastid division site position between stroma and outer surface via interactions with the stromal FtsZ ring and the outer membrane PDV2 that recruits cytoplasmic ARC5 ring. Required for plastid equatorial positioning of PDV2 and ARC5. May contribute to gravitropism in stems and hypocotyls. Seems to influence stromule (stroma-filled tubular extensions of the plastid envelope membrane) length and frequency.
Gene References into Functions
  1. The ARC6 intermembrane space region bears a structurally uncharacterized domain of unknown function, DUF4101, that is highly conserved among ARC6 and Ftn2 proteins. This study reports the crystal structure of this domain. PMID: 26452626
  2. These results suggest that besides ARC6-mediated anchoring, an additional FtsZ-anchoring mechanism is present in chloroplasts. PMID: 23578755
  3. The effect of reduced chloroplast number on the photosynthetic competence of leaves of A. thaliana with mutations at accumulation and replication of chloroplasts (ARC) sites ARC3, ARC5, and ARC6 are reported. PMID: 16170638
  4. alteration in stromule length in arc3 and arc6 mutants occurred despite wild-type plastid densities PMID: 18764889
Subcellular Location
Plastid, chloroplast inner membrane; Single-pass membrane protein. Note=Localized to a ring at the center of the chloroplasts (equatorial positioning) prior to and during constriction.
Database Links

KEGG: ath:AT5G42480

STRING: 3702.AT5G42480.1

UniGene: At.30169

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