Recombinant Arabidopsis thaliana Protein BRASSINAZOLE-RESISTANT 1 (BZR1)

Code CSB-YP854652DOA
MSDS
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Source Yeast
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Code CSB-EP854652DOA
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Source E.coli
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Code CSB-EP854652DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP854652DOA
MSDS
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Source Baculovirus
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Code CSB-MP854652DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BZR1
Uniprot No.
Alternative Names
BZR1; BIS2; At1g75080; F9E10_7Protein BRASSINAZOLE-RESISTANT 1; Protein BIN2 SUBSTRATE 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-336
Target Protein Sequence
MTSDGATSTS AAAAAAAAAA ARRKPSWRER ENNRRRERRR RAVAAKIYTG LRAQGDYNLP KHCDNNEVLK ALCVEAGWVV EEDGTTYRKG CKPLPGEIAG TSSRVTPYSS QNQSPLSSAF QSPIPSYQVS PSSSSFPSPS RGEPNNNMSS TFFPFLRNGG IPSSLPSLRI SNSCPVTPPV SSPTSKNPKP LPNWESIAKQ SMAIAKQSMA SFNYPFYAVS APASPTHRHQ FHTPATIPEC DESDSSTVDS GHWISFQKFA QQQPFSASMV PTSPTFNLVK PAPQQMSPNT AAFQEIGQSS EFKFENSQVK PWEGERIHDV GMEDLELTLG NGKARG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional repressor that binds to the brassinosteroid (BR) response element (BRRE) 5'-CGTG(T/C)G-3' in gene promoter. Regulates positively the brassinosteroid-signaling pathway. Mediates downstream growth responses and negative feedback regulation of brassinosteroid biosynthesis. Promotes growth. Modulates ovule initiation and development by monitoring the expression of genes related to ovule development (e.g. HLL, ANT, and AP2).
Gene References into Functions
  1. The brassinosteroid-regulated transcription factors BZR1/BES1 function as a coordinator in multisignal-regulated plant growth. PMID: 29673687
  2. light-regulated phosphorylation change of BZR1 requires the existence of endogenous brassinosteroid as well as functional BIN2 and protein phosphatase 2A (PP2A). PMID: 28396515
  3. BZR1, a BES1 homologue, promotes cell divisions in the QC, but it suppresses columella stem cell differentiation, opposite to the action of BES1. PMID: 26136267
  4. BZR1 represses target genes by recruiting the Groucho/TUP1-like transcriptional corepressor TOPLESS. PMID: 24938363
  5. BZR1 acts as an important regulator mediating the trade-off between growth and immunity upon integration of environmental cues PMID: 24381244
  6. BIL1/BZR1 increases plant resistance to insect feeding PMID: 25036120
  7. BZR1-interacting proteins have been identified as potential components of the brassinosteroid signaling pathway in Arabidopsis. PMID: 24019147
  8. Phosphatidic acid decreased BZR1 dephosphorylation to inhibit BZR1 activity and brassinosteroid signal response by regulating PP2A activity. PMID: 24121289
  9. BZR1 and AP2 probably affect Arabidopsis ovule number determination antagonistically PMID: 22914576
  10. BZR1 dissociates from the DWF4 (cytochrome P450 90B1) promoter in the presence of auxin, indicating DWF4 expression involves BZR1-mediated de-repression mechanisms. PMID: 21284753
  11. Data show that BZR1 not only controls the expression of many signaling components of other hormonal and light pathways but also coregulates common target genes with light-signaling transcription factors. PMID: 21074725
  12. results show that BZR1 coordinates brassinosteroid (BR) homeostasis and signaling by playing dual roles in regulating BR biosynthesis and downstream growth responses PMID: 15681342
  13. BZR1 functions as a nucleocytoplasmic shuttling protein and GSK3-like kinases induce the nuclear export of BZR1 by modulating BZR1 interaction with the 14-3-3 proteins. PMID: 17873094

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Subcellular Location
Nucleus.
Protein Families
BZR/LAT61 family
Tissue Specificity
Accumulates in the growing region of the hypocotyl.
Database Links

KEGG: ath:AT1G75080

STRING: 3702.AT1G75080.1

UniGene: At.17945

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