Recombinant Arabidopsis thaliana Protein BRASSINAZOLE-RESISTANT 2 (BZR2)

Code CSB-YP888760DOA
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Source Yeast
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Code CSB-EP888760DOA
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Source E.coli
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Code CSB-EP888760DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP888760DOA
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Source Baculovirus
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Code CSB-MP888760DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BZR2
Uniprot No.
Alternative Names
BZR2; BES1; BIS1; At1g19350; F18O14_4Protein BRASSINAZOLE-RESISTANT 2; Protein 107; Protein BIN2 SUBSTRATE 1; Protein BRI1-EMS-SUPPRESSOR 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-335
Target Protein Sequence
MTSDGATSTS AAAAAAAMAT RRKPSWRERE NNRRRERRRR AVAAKIYTGL RAQGNYNLPK HCDNNEVLKA LCSEAGWVVE EDGTTYRKGH KPLPGDMAGS SSRATPYSSH NQSPLSSTFD SPILSYQVSP SSSSFPSPSR VGDPHNISTI FPFLRNGGIP SSLPPLRISN SAPVTPPVSS PTSRNPKPLP TWESFTKQSM SMAAKQSMTS LNYPFYAVSA PASPTHHRQF HAPATIPECD ESDSSTVDSG HWISFQKFAQ QQPFSASMVP TSPTFNLVKP APQQLSPNTA AIQEIGQSSE FKFENSQVKP WEGERIHDVA MEDLELTLGN GKAHS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Positive regulator of brassinosteroid (BR) signaling. Transcription factor that activates target gene expression by binding specifically to the DNA sequence 5'-CANNTG-3'(E box) through its N-terminal domain. Can bind individually to the promoter as a homodimer or synergistically as a heterodimer with BIM1, BIM2 or BIM3. The C-terminal domain is probably involved in transcriptional activation. Recruits the transcription elongation factor IWS1 to control BR-regulated gene expression. Forms a trimeric complex with IWS1 and ASHH2/SDG8 to regulate BR-regulated gene expression. Promotes quiescent center (QC) self-renewal by cell divisions in the primary root. Binds to the E-boxes of the BRAVO promoter to repress its expression.
Gene References into Functions
  1. BES1 and BZR1 are key regulators of both xylem and phloem cell differentiation from vascular stem cells. PMID: 29385529
  2. BES1 and BZR1 are brassinolide-targeted genes in Arabidopsis thaliana. PMID: 28124247
  3. regulates the localization of the brassinosteroid receptor BRL3 within the provascular tissue of primary root PMID: 27511026
  4. SINA of Arabidopsis thaliana (SINATs) specifically interact with dephosphorylated BES1 and mediate its ubiquitination and degradation. PMID: 28399399
  5. BES1-L is a more important isoform of BES1 in Arabidopsis and may have contributed to the evolution and expansion of A. thaliana. PMID: 25649439
  6. propose that the environmentally controlled developmental switch from deep to shallow root architecture involves reductions in BZR1 and BES1/BZR2 levels in the nucleus, which likely play key roles in plant adaptation to phosphate-deficient environments PMID: 25136063
  7. role in regulation of glucosinolate biosynthesis by brassinosteroids PMID: 23580754
  8. altered patterns of BES1 phosphorylation in roc1-1D, roc1-2 and roc1-3 mutants PMID: 22463079
  9. BES1 directly regulates many brassinosteroid-responsive genes by initiating a hierarchical transcription network downstream of BR signaling. PMID: 21214652
  10. BES1 is a nucleocytoplasmic signal transmitter. Its subcellular localization modulates brassinosteroid signal output intensity. Phosphorylation at 12 sites induces its nuclear export by regulating its binding affinity with 14-3-3 proteins. PMID: 20387034
  11. Cytosolic BSU1 and nuclear BIN2 might be required for the efficient subcellular localization of BES1 in BR signaling. PMID: 20387035
  12. AtIWS1 is recruited to target genes by BES1 to promote gene expression during transcription elongation process. PMID: 20139304

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
BZR/LAT61 family
Tissue Specificity
Ubiquitously expressed in cotyledons, leaves, hypocotyls and roots.
Database Links

KEGG: ath:AT1G19350

STRING: 3702.AT1G19350.3

UniGene: At.24276

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