Recombinant Arabidopsis thaliana Protein COBRA (COB)

Code CSB-YP836029DOA
MSDS
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Source Yeast
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Code CSB-EP836029DOA
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Source E.coli
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Code CSB-EP836029DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP836029DOA
MSDS
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Source Baculovirus
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Code CSB-MP836029DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
COB
Uniprot No.
Alternative Names
COB; At5g60920; MSL3.40Protein COBRA; Cell expansion protein
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
37-431
Target Protein Sequence
YDAL DPEGNITMKW DVMSWTPDGY VAVVTMFNFQ KYRHIQSPGW TLGWKWAKKE VIWSMVGAQT TEQGDCSKYK GNIPHCCKKD PTVVDLLPGT PYNQQIANCC KGGVMNSWVQ DPATAASSFQ ISVGAAGTTN KTVRVPRNFT LMGPGPGYTC GPAKIVRPTK FVTTDTRRTT QAMMTWNITC TYSQFLAQRT PTCCVSLSSF YNETIVGCPT CACGCQNNRT ESGACLDPDT PHLASVVSPP TKKGTVLPPL VQCTRHMCPI RVHWHVKQNY KEYWRVKITI TNFNYRLNYT QWNLVAQHPN LDNITQIFSF NYKSLTPYAG LNDTAMLWGV KFYNDFLSEA GPLGNVQSEI LFRKDQSTFT FEKGWAFPRR IYFNGDNCVM PPPDSYPFLP N
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in determining the orientation of cell expansion, probably by playing an important role in cellulose deposition. May act by recruiting cellulose synthesizing complexes to discrete positions on the cell surface.
Gene References into Functions
  1. COBRA's function in maintaining anisotropic expansion may be microtubule-dependent. PMID: 16284708
  2. Mutations within the COBRA gene that result in a loss of function result in abnormal cell growth throughout the entire plant body and the accumulation of massive amounts of stress response chemicals such as anthocyanins and callose. PMID: 16873454
Subcellular Location
Lateral cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Note=Located on the longitudinal sides of the cell rather than on the apical or basal sides.
Protein Families
COBRA family
Tissue Specificity
Expressed in roots, stems, leaves, flowers and siliques. Up-regulated in the root zone of rapid longitudinal expansion.
Database Links

KEGG: ath:AT5G60920

STRING: 3702.AT5G60920.1

UniGene: At.21375

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