Recombinant Arabidopsis thaliana Protein PROTON GRADIENT REGULATION 5, chloroplastic (PGR5)

Code CSB-YP879779DOA
MSDS
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Source Yeast
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Code CSB-EP879779DOA
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Source E.coli
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Code CSB-EP879779DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP879779DOA
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Source Baculovirus
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Code CSB-MP879779DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PGR5
Uniprot No.
Alternative Names
PGR5; At2g05620; T20G20.3Protein PROTON GRADIENT REGULATION 5; chloroplastic
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
45-133
Target Protein Sequence
QQARVS RKAIRAVPMM KNVNEGKGLF APLVVVTRNL VGKKRFNQLR GKAIALHSQV ITEFCKSIGA DAKQRQGLIR LAKKNGERLG FLA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the regulation of the cyclic electron flow (CEF) around Photosystem I. Essential for the reduction of PGRL1A by ferredoxin and for photoprotection.
Gene References into Functions
  1. The results indicate that DeltapH formation is mostly due to linear electron flow (LEF), with the contributions of PGR5- and NDH-dependent cyclic electron flow (CEF) estimated as only ~30 and ~5%, respectively. PMID: 28828567
  2. PGR5-PGRL1-mediated cyclic electron transport plays a major role in DeltapH formation and induction of non-photochemical quenching. PMID: 26687812
  3. PGR5 is not involved in non-photochemical reduction of plastoquinone pool and state transitions in Arabidopsis thaliana. PMID: 25792151
  4. The simulation also suggested a role of the PGR5-dependent cyclic electron flow (CEF). in accelerating electron transfer in the cytochrome b6f complex. PMID: 24725611
  5. Data indicate that photosystem II (PSII) yield was almost fully resistant to antimycin A in leaves accumulating endogenous levels of PtPGR5 or AtPGR5 V3K that had lysine instead of valine at the third position. PMID: 23872270
  6. PROTON GRADIENT REGULATION5 (PGR5)-dependent regulation of electron transfer and proton motive force is crucial for protection of photosystem I against photodamage, which occurred particularly during the high light phases of fluctuating light cycles. PMID: 22822205
  7. pgr5 mutant is affected in the redox poising of the chloroplast PMID: 17803955
  8. This study suggested that a balanced PGR5 level is required for efficient regulation of the rate of antimycin A-sensitive photosystem I cyclic electron transport. PMID: 17913767
  9. A complex containing PGRL1 and PGR5 is involved in the switch between linear and cyclic electron flow in Arabidopsis. PMID: 18243102
  10. These results suggest that the rate of PGR5-dependent photosystem I cyclic electron transport is high enough to balance the production ratio of ATP and NADPH during steady-state photosynthesis, consistently with the pgr5 mutant phenotype. PMID: 18388110
  11. PGR5 has a developmentally-regulated, conserved role in mediating cyclic electron transport. PMID: 18663471
  12. PGR5 mutants show a reduction of photosynthesis and growth, which results from ATP deficiency and inactivation of PSI. PMID: 18799484

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Subcellular Location
Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side.
Protein Families
PGR5 family
Database Links

KEGG: ath:AT2G05620

STRING: 3702.AT2G05620.1

UniGene: At.27446

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