Recombinant Arabidopsis thaliana Protein-S-isoprenylcysteine O-methyltransferase A (ICMTA), partial

Code CSB-YP866990DOA1
MSDS
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Source Yeast
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Code CSB-EP866990DOA1
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Source E.coli
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Code CSB-EP866990DOA1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866990DOA1
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Source Baculovirus
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Code CSB-MP866990DOA1
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ICMTA
Uniprot No.
Alternative Names
ICMTA; PCM; STE14; STE14A; At5g23320; MKD15.18; Protein-S-isoprenylcysteine O-methyltransferase A; AtICMTA; Isoprenylcysteine carboxylmethyltransferase A; Prenylated protein carboxyl methyltransferase A; Prenylcysteine carboxyl methyltransferase A; AtPCM
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues, resulting in the modulation of the function of prenylated proteins. Involved in negative regulation of abscisic acid signaling. Carboxyl methylation is a reversible and potentially regulated step in the post-translational modification of prenylated proteins.
Gene References into Functions
  1. Observations support the hypothesis that the ABA hypersensitive phenotype of fcly plants is the result of FC accumulation and inhibition of ICMT. PMID: 19969520
  2. phenotype of RNAi lines is similar to farnesyltransferase beta-subunit mutant enhanced response to abscisic acid2 but is more subtle PMID: 18641086
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
Class VI-like SAM-binding methyltransferase superfamily, Isoprenylcysteine carboxyl methyltransferase family
Tissue Specificity
Expressed primarily in flowers, stems, leaves and roots. Almost not expressed in siliques. Detected in root tips and vascular tissues of roots, cotyledons, petiols, hypocotyls, filaments, pollen grains and the distal and proximal portions of the gynoecium
Database Links

KEGG: ath:AT5G23320

STRING: 3702.AT5G23320.1

UniGene: At.54958

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