Recombinant Arabidopsis thaliana Protein STRUBBELIG (SUB), partial

Code CSB-YP840427DOA
MSDS
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Source Yeast
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Code CSB-EP840427DOA
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Source E.coli
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Code CSB-EP840427DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP840427DOA
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Source Baculovirus
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Code CSB-MP840427DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SUB
Uniprot No.
Alternative Names
SUB; SCM; At1g11130; T19D16.1; T19D16.8; T28P6.18; Protein STRUBBELIG; Leucine-rich repeat receptor kinase-like protein SUB; Protein SCRAMBLED
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulates the expression of transcription factors that define the cell fates. Acts in a non-cell-autonomous fashion, functions in a radial inside-out signaling process, and mediates cell morphogenesis and cell fate across clonally distinct cell layers in floral primordia, developing ovules, and root meristems. Seems to be required for the regulation of cell shape and the orientation of the mitotic division plane. Involved in root hair specification, in the formation of the outer integument and the shape of organs such as carpels and petals and is necessary for the shape and height of the stem. Non-functional SUB proteins are retained in the endoplasmic reticulum and degraded by endoplasmic reticulum-associated degradation (ERAD).
Gene References into Functions
  1. SUB is mis-targeted in hap13-1. However, unlike that of PIN2, the distribution of PIN1 is independent of HAP13. Genetic interference of exocytic trafficking at the TGN/EE by specifically downregulating HAP13 phenocopied the defects of hap13-1 in SUB targeting and ovule development. PMID: 27541731
  2. These results suggest that the AN protein is one of the critical downstream factors of SCM pathways specifically responsible for mediating its effects on cell/tissue morphogenesis through cortical microtubule arrangement. PMID: 26296462
  3. importance of distinct kinase residues for these three developmental processes provides an explanation for the maintenance of the conserved kinase domain in the SCM protein PMID: 25136062
  4. STRUBBELIG extracellular domain variant harbours considerable amounts of Glc1Man7GlcNAc2 N-glycans suggesting that the ER-quality control processes are tightly interconnected to promote protein folding or disposal. PMID: 25251695
  5. The C2-domain protein QUIRKY and the receptor-like kinase STRUBBELIG localize to plasmodesmata and mediate tissue morphogenesis in Arabidopsis thaliana PMID: 25256344
  6. QKY is localized at the plasma membrane and functions in a multiprotein complex that includes SUB and PAL OF QUIRKY, a previously uncharacterized PB1-domain-containing protein that localizes both at the plasma membrane and in intracellular compartments. PMID: 24173806
  7. ANGUSTIFOLIA is a central component of tissue morphogenesis mediated by the atypical receptor-like kinase STRUBBELIG. PMID: 23368817
  8. SCM/SUB mediates developmental stage-specific signal for leaf patterning PMID: 22805005
  9. Studying SUB-dependent signalling will contribute to our understanding of how atypical kinases mediate signal transduction and how cells co-ordinate their behaviour to allow organs, such as ovules, to develop their three-dimensional architecture. PMID: 20298225
  10. a new gene, SCRAMBLED, is described; required for cells to appropriately interpret their location within the developing root epidermis [SCRAMBLED][SCM] PMID: 15618487
  11. These findings help define the developmental and mechanistic role of SCRAMBLED and suggest a new model for its action in root epidermal cell patterning. PMID: 17027738
  12. SUB acts in a non-cell-autonomous fashion, functions in a radial inside-out signaling process, and mediates cell morphogenesis and cell fate across clonally distinct cell layers in floral primordia, developing ovules, and root meristems. PMID: 18771664
  13. SCM expression and accumulation in the epidermal cell layer is necessary and sufficient to direct the cell-type pattern. PMID: 19097902
  14. DOQ, QKY, and ZET contribute to SUB-dependent organogenesis. PMID: 19180193

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Subcellular Location
Cell membrane; Single-pass membrane protein.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Tissue Specificity
Expressed in leaves, stems, inflorescences, flower buds and developing root epidermis.
Database Links

KEGG: ath:AT1G11130

STRING: 3702.AT1G11130.1

UniGene: At.42148

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