Recombinant Arabidopsis thaliana Protein TRANSPARENT TESTA GLABRA 1 (TTG1)

Code CSB-YP893569DOA
MSDS
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Source Yeast
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Code CSB-EP893569DOA
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Source E.coli
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Code CSB-EP893569DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP893569DOA
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Source Baculovirus
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Code CSB-MP893569DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TTG1
Uniprot No.
Alternative Names
TTG1; At5g24520; K18P6_4; T31K7.8; Protein TRANSPARENT TESTA GLABRA 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-341
Target Protein Sequence
MDNSAPDSLS RSETAVTYDS PYPLYAMAFS SLRSSSGHRI AVGSFLEDYN NRIDILSFDS DSMTVKPLPN LSFEHPYPPT KLMFSPPSLR RPSSGDLLAS SGDFLRLWEI NEDSSTVEPI SVLNNSKTSE FCAPLTSFDW NDVEPKRLGT CSIDTTCTIW DIEKSVVETQ LIAHDKEVHD IAWGEARVFA SVSADGSVRI FDLRDKEHST IIYESPQPDT PLLRLAWNKQ DLRYMATILM DSNKVVILDI RSPTMPVAEL ERHQASVNAI AWAPQSCKHI CSGGDDTQAL IWELPTVAGP NGIDPMSVYS AGSEINQLQW SSSQPDWIGI AFANKMQLLR V
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May regulate MYC transcription factors. Involved in epidermal cell fate specification such as trichome and root hair development, seed mucilage production, and anthocyanin biosynthesis by acting at the dihydroflavonol-4-reductase (DFR) step. Together with GL1 and GL3, promotes trichome formation. Activates the transcription of GL2.
Gene References into Functions
  1. TTG1 is a direct target of FUS3 in the framework of the regulatory hierarchy controlling seed filling. PMID: 26152712
  2. Lateral root following abscisic acid treatment was reduced in ttg1 seedlings and increased in plants overexpressing TTG1. PMID: 23306631
  3. TTG1 trapping by GL3 is based on direct interaction between both proteins and recruitment in the nucleus. PMID: 22028035
  4. TTG1 is involved in the regulation of TT8 expression. PMID: 16709193
  5. This study showed that overexpression of Myb113 or Myb114 results in substantial increases in pigment production, and that pigment production remains TTG1- and bHLH-dependent. PMID: 18036197
  6. Data show that TTG1-bHLH-MYB complex directly regulating activators and repressors and the movement of repressors to affect trichome patterning on the Arabidopsis leaf. PMID: 18434419
  7. the role of TTG1 in Arabidopsis trichome formation PMID: 18547143
  8. phytohormonal control is mediated by transcriptional regulation of the established TTG1 complex and depended on the R2R3-MYBB factor GLABRA1 PMID: 18784284
  9. Although the TTG1-dependent R2R3 MYBs are considered highly pathway specific, identification of MYBs responsible for outer seed coat development allowed for the elucidation of previously undetected novel developmental pleiotropy among these elements. PMID: 18992236

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Subcellular Location
Cytoplasm.
Tissue Specificity
Roots, leaves, stems, meristems, flowers and flower buds.
Database Links

KEGG: ath:AT5G24520

STRING: 3702.AT5G24520.1

UniGene: At.28124

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