Recombinant Arabidopsis thaliana Rop guanine nucleotide exchange factor 2 (ROPGEF2)

Code CSB-YP862843DOA
MSDS
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Source Yeast
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Code CSB-EP862843DOA
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Source E.coli
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Code CSB-EP862843DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP862843DOA
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Source Baculovirus
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Code CSB-MP862843DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ROPGEF2
Uniprot No.
Alternative Names
ROPGEF2; At1g01700; T1N6.8; Rop guanine nucleotide exchange factor 2; AtRopGEF2; Rho of plants guanine nucleotide exchange factor 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-485
Target Protein Sequence
MENLPNHEEN DDVGYHQSPG PIDPNDHSAS ETPVYSTMST DSFAYHRTCS ETSGGGFSDQ IDETSSFCTE ASPSDWPVLT ESNNSASSNF PTVFDLKHNQ IETDEHLAVQ EISEPELETM KERFSKLLLG EDMSGSGKGV CTAVTISNAI TNLYATVFGQ NLRLEPLEIE QKTTWKREMN CLLSVCDYIF EFIPKSQNLS NGATVEVMES RPRADIYINL PALRKLDSML MEALDSFQKT EFWYAEEGSL SMKSTRSATG SFRKVIVQRK EEKWWLPIPL VPLQGLSEKA RKQLKSKRES TNQIHKAAMA INSSILGEMD IPDSYMATLP KSGKASTGDA IYRHMTSSGR FSPEKLLDRL KIVSEHEALQ LADRVEASMY TWRRKACLNN SKSSWNMVKD LMSITERSDK NYVLAERAES LLFCLKQRYP ELSQTSLDIC KIHCNKDVGK AVLESYSRVL EGLAFNIVAW IDDVLYVDKT MRGEE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Guanine-nucleotide exchange factor (GEF) that acts as an activator of Rop (Rho of plants) GTPases by promoting the exchange of GDP for GTP.
Gene References into Functions
  1. RopGEF2 acted as a negative regulator in phytochrome B (phyB)-mediated red light-induced stomatal opening.RopGEF2 interacted with ROP7 and ROP2 and enhanced their intrinsic nucleotide exchange rates. PMID: 28188273
  2. Disruption of RopGEF2 enhances sensitivity to exogenous ABA in seed germination assays and that RopGEF2pro-GUS is mainly expressed in developing embryos and germinating seeds. PMID: 26461226
Tissue Specificity
Expressed in the vascular tissues of roots, leaves, sepals, petals and siliques.
Database Links
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