Recombinant Arabidopsis thaliana Serine/threonine-protein kinase Aurora-3 (AUR3)

Code CSB-YP528946DOA
MSDS
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Source Yeast
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Code CSB-EP528946DOA
MSDS
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Source E.coli
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Code CSB-EP528946DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP528946DOA
MSDS
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Source Baculovirus
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Code CSB-MP528946DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AUR3
Uniprot No.
Alternative Names
AUR3; At2g45490; F17K2.2Serine/threonine-protein kinase Aurora-3; AtAur3; EC 2.7.11.1; Aurora-like kinase 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-288
Target Protein Sequence
MSKKSTESDA GNTEKQWSLA DFEIGRPLGK GKFGRVYLAR EAKSKYIVAL KVIFKEQIEK YKIHHQLRRE MEIQTSLRHP NILRLFGWFH DNERIFLILE YAHGGELYGV LKQNGHLTEQ QAATYIASLS QALAYCHGKC VIHRDIKPEN LLLDHEGRLK IADFGWSVQS SNKRKTMCGT LDYLAPEMVE NRDHDYAVDN WTLGILCYEF LYGNPPFEAE SQKDTFKRIL KIDLSFPLTP NVSEEAKNLI SQLLVKDPSK RLSIEKIMQH PWIVKNADPK GVCASIDI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Phosphorylates in vitro histone H3 at 'Ser-10' (H3S10ph) and 'Ser-28' (H3S28ph), but not at 'Thr-3' (H3T3ph) or 'Thr-11' (H3T11ph). Colocalizes with phosphorylated histone H3 during mitosis. Associates with cytoskeletal structures that are necessary for cytokinesis and with the microtubule spindle.
Gene References into Functions
  1. AtAUR1 and AtAUR3 have both redundant and distinct functions through interactions with transcription factors (TFs). In addition, database analysis revealed that most of the highly AtAUR-interacting TFs contained a detectable phosphopeptide that was consistent with the consensus motifs for human AURs, suggesting that these TFs are substrates of the AtAURs. PMID: 27734173
  2. Aurora kinase AtAUR3 showed dot-like distribution on condensed chromosomes at prophase and then localized at the metaphase plate, and overexpression of AtAUR3 induces disassembly of spindle microtubules and alteration of orientation of cell division. PMID: 16028112
Subcellular Location
Cytoplasm, perinuclear region. Nucleus. Chromosome. Chromosome, centromere.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, Aurora subfamily
Tissue Specificity
Abundant in roots, flowers and flower buds, low or absent in expanded leaves, stems and siliques.
Database Links

KEGG: ath:AT2G45490

STRING: 3702.AT2G45490.1

UniGene: At.37011

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