Recombinant Arabidopsis thaliana Serine/threonine-protein phosphatase PP2A-5 catalytic subunit (PP2A5)

Code CSB-YP520810DOA
MSDS
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Source Yeast
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Code CSB-EP520810DOA
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Source E.coli
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Code CSB-EP520810DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP520810DOA
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Source Baculovirus
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Code CSB-MP520810DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PP2A5
Uniprot No.
Alternative Names
PP2A5; At1g69960; F20P5.30; T17F3.1; Serine/threonine-protein phosphatase PP2A-5 catalytic subunit; EC 3.1.3.16; Protein phosphatase 2A isoform 5
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-307
Target Protein Sequence
MPPATGDIDR QIEQLMECKA LSETEVKMLC EHAKTILVEE YNVQPVKCPV TVCGDIHGQF YDLIELFRIG GSSPDTNYLF MGDYVDRGYY SVETVSLLVA LKVRYRDRLT ILRGNHESRQ ITQVYGFYDE CLRKYGNANV WKHFTDLFDY LPLTALIESQ VFCLHGGLSP SLDTLDNIRS LDRIQEVPHE GPMCDLLWSD PDDRCGWGIS PRGAGYTFGQ DIATQFNHTN GLSLISRAHQ LVMEGFNWCQ EKNVVTVFSA PNYCYRCGNM AAILEIGENM DQNFLQFDPA PRQVEPETTR KTPDYFL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Associates with the serine/threonine-protein phosphatase PP2A regulatory subunits A and B' to positively regulates beta-oxidation of fatty acids and protoauxins in peroxisomes by dephosphorylating peroxisomal beta-oxidation-related proteins. Involved in the positive regulation of salt stress responses. May function by increasing chloride channel activities on vacuolar membranes.
Gene References into Functions
  1. data indicate that PP2A-C5-mediated better growth under salt conditions might involve up-regulation of CLC activities on vacuolar membranes and that PP2A-C5 could be used for improving salt tolerance in crops. [PP2A-C5] PMID: 27676158
  2. The PP2A inhibitor phosphorylated alpha-endosulfine (ENSA)/ARPP-19 is highly conserved across plant species. ENSA/ARPP-19 is only expressed in a selection of Arabidopsis thaliana tissues. PMID: 25666948
  3. Phosphatidic acid interacts with the scaffolding A1 subunit of Protein Phosphatase 2A (PP2A) and regulates PP2A-mediated PIN1 dephosphorylation in Arabidopsis. PMID: 23686948
  4. PP2A exerts multilevel control on HMGR through the five-member B'' protein family during normal development and in response to a variety of stress conditions. PMID: 21478440
  5. PP2A directly interacts with the ACS6 protein, and PP2A complexes dephosphorylate a carboxy-terminal ACS6 phosphopeptide in vitro. PMID: 21533019
  6. Data reveal a crucial function for PP2A in dephosphorylating and activating BZR1 and completes the set of core components of the brassinosteroid-signalling cascade from cell surface receptor kinase to gene regulation in the nucleus. PMID: 21258370

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Subcellular Location
Cytoplasm, cytosol. Peroxisome.
Protein Families
PPP phosphatase family, PP-2A subfamily
Database Links

KEGG: ath:AT1G69960

STRING: 3702.AT1G69960.1

UniGene: At.20106

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