Recombinant Arabidopsis thaliana Sucrose-phosphate synthase 1 (SPS1), partial

Code CSB-EP856727DOA
MSDS
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
SPS1
Uniprot No.
Research Area
Signal Transduction
Alternative Names
SPS1; SPSA1; At5g20280; F5O24.170; Sucrose-phosphate synthase 1; EC 2.4.1.14; Sucrose-phosphate synthase 1F; AtSPS1F; Sucrose-phosphate synthase 5.1; AtSPS5.1; UDP-glucose-fructose-phosphate glucosyltransferase
Species
Arabidopsis thaliana (Mouse-ear cress)
Source
E.coli
Expression Region
768-995aa
Target Protein Sequence
VVIALDFDGEEDTLEATKRILDAVEKERAEGSVGFILSTSLTISEVQSFLVSGGLNPNDFDAFICNSGSDLHYTSLNNEDGPFVVDFYYHSHIEYRWGGEGLRKTLIRWASSLNEKKADNDEQIVTLAEHLSTDYCYTFTVKKPAAVPPVRELRKLLRIQALRCHVVYSQNGTRINVIPVLASRIQALRYLFVRWGIDMAKMAVFVGESGDTDYEGLLGGLHKSVVLK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
41.5kDa
Protein Length
Partial
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a major role in photosynthetic sucrose synthesis by catalyzing the rate-limiting step of sucrose biosynthesis from UDP-glucose and fructose- 6-phosphate. Involved in the regulation of carbon partitioning in the leaves of plants. May regulate the synthesis of sucrose and therefore play a major role as a limiting factor in the export of photoassimilates out of the leaf. Plays a role for sucrose availability that is essential for plant growth and fiber elongation. Required for nectar secretion.
Gene References into Functions
  1. Regulation of sucrose-phosphate synthase by BSK8 occurs through activation of a phosphatase that in turn may dephosphorylate sucrose-phosphate synthase and thus activates the enzyme. PMID: 24924143
  2. SPSA1 plays a role in photosynthetic sucrose synthesis in Arabidopsis leaves. Decreases in leaf SPS activity lead to increased starch synthesis and turnover, and decreased RuBP regeneration-limited photosynthesis but not Rubisco-limited photosynthesis. PMID: 21309792
Protein Families
Glycosyltransferase 1 family
Tissue Specificity
Expressed in seeds, stems, rosette leaves, flowers and siliques. Highly expressed in maturing nectaries.
Database Links

KEGG: ath:AT5G20280

STRING: 3702.AT5G20280.1

UniGene: At.22681

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