Recombinant Arabidopsis thaliana Transcription factor MYB46 (MYB46)

Code CSB-YP888858DOA
MSDS
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Source Yeast
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Code CSB-EP888858DOA
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Source E.coli
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Code CSB-EP888858DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP888858DOA
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Source Baculovirus
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Code CSB-MP888858DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MYB46
Uniprot No.
Alternative Names
MYB46; At5g12870; T24H18.40; Transcription factor MYB46; Myb-related protein 46; AtMYB46
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-280
Target Protein Sequence
MRKPEVAIAA STHQVKKMKK GLWSPEEDSK LMQYMLSNGQ GCWSDVAKNA GLQRCGKSCR LRWINYLRPD LKRGAFSPQE EDLIIRFHSI LGNRWSQIAA RLPGRTDNEI KNFWNSTIKK RLKKMSDTSN LINNSSSSPN TASDSSSNSA SSLDIKDIIG SFMSLQEQGF VNPSLTHIQT NNPFPTGNMI SHPCNDDFTP YVDGIYGVNA GVQGELYFPP LECEEGDWYN ANINNHLDEL NTNGSGNAPE GMRPVEEFWD LDQLMNTEVP SFYFNFKQSI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription activator. Involved in the regulation of secondary wall biosynthesis in fibers and vessels. Transcription activator of the mannan synthase CSLA9 that recognizes and binds to the DNA consensus sequence 5'-[AG][GT]T[AT]GGT[GA]-3' cis-regulatory element of CSLA9 promoter. Transcription factor that acts as molecular switch in the NAC012/SND1-mediated transcriptional network regulating secondary wall biosynthesis. Is directly activated by NAC012/SND1. Functions redundantly with MYB83 in the transcriptional regulatory cascade leading to secondary wall formation in fibers and vessels. Transcription activator that binds to the DNA consensus sequence 5'-ACC[AT]A[AC][TC]-3', designated as the secondary wall MYB-responsive element (SMRE). Regulates directly numerous transcription factors and a number of genes involved in secondary wall biosynthesis that contain SMRE elements in their promoters. Is an obligate component of the transcriptional regulatory complex toward the commitment of secondary wall cellulose synthesis. Is required for functional expression of the three secondary wall CESA genes, CESA4, CESA7 and CESA8.
Gene References into Functions
  1. directly regulates all three secondary wall-associated cellulose synthase genes PMID: 26011122
  2. MYB46 is a unique transcription factor in that it directly regulates the biosynthesis genes for all of the three major components of the secondary wall as well as the transcription factors in the biosynthesis pathway. PMID: 24984711
  3. MYB46 functions as a central and direct regulator of the genes involved in the biosynthesis of all three major secondary wall components. PMID: 24879533
  4. Data conclude that MYB46 is an obligate component of the transcriptional regulatory complex toward the commitment of secondary wall cellulose synthesis in Arabidopsis. PMID: 23726771
  5. MYB46 mediated the transcriptional regulation of secondary wall biosynthesis. PMID: 22914575
  6. MYB46 activates the secondary wall MYB-responsive element sites in the promoters of MYB46 direct targets. MYB46 and MYB83 directly activate a set of downstream transcription factors. PMID: 22197883
  7. MYB46 recognizes a cis-acting regulatory motif M46RE PMID: 22271306
  8. The observed coordinated down-regulation of CesA genes is more pronounced, and occurs earlier, in myb46 mutant plants. PMID: 21617373
  9. MYB46 gene expression does not change following interaction with pathogens. myb46 mutants have enhanced disease resistance to B. cinerea. PMID: 21282403
  10. MYB46 activates the expression of selected transcription factors and genes involved in the biosynthesis of secondary wall components. PMID: 19674407
  11. These results demonstrate that MYB46 is a direct target of SND1 and is another key player in the transcriptional network involved in the regulation of secondary wall biosynthesis in Arabidopsis. PMID: 17890373

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed at low levels in stems and siliques, specifically in xylem.
Database Links

KEGG: ath:AT5G12870

STRING: 3702.AT5G12870.1

UniGene: At.5283

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