Recombinant Arabidopsis thaliana Transcription factor MYC2 (MYC2), partial

Code CSB-YP657884DOA
MSDS
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Source Yeast
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Code CSB-EP657884DOA
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Source E.coli
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Code CSB-EP657884DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP657884DOA
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Source Baculovirus
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Code CSB-MP657884DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MYC2
Uniprot No.
Alternative Names
MYC2; BHLH6; EN38; JAI1; JIN1; RAP1; RD22BP1; ZBF1; At1g32640; F6N18.4; Transcription factor MYC2; AtMYC2; Basic helix-loop-helix protein 6; AtbHLH6; bHLH 6; Protein JASMONATE INSENSITIVE 1; R-homologous Arabidopsis protein 1; RAP-1; Transcription factor EN 38; Z-box binding factor 1 protein; bHLH transcription factor bHLH006; rd22BP1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator. Common transcription factor of light, abscisic acid (ABA), and jasmonic acid (JA) signaling pathways. With MYC3 and MYC4, controls additively subsets of JA-dependent responses. In cooperation with MYB2 is involved in the regulation of ABA-inducible genes under drought stress conditions. Can form complexes with all known glucosinolate-related MYBs to regulate glucosinolate biosynthesis. Binds to the MYC recognition site (5'-CACATG-3'), and to the G-box (5'-CACNTG-3') and Z-box (5'-ATACGTGT-3') of promoters. Binds directly to the promoters of the transcription factors PLETHORA1 (PLT1) and PLT2 and represses their expression. Negative regulator of blue light-mediated photomorphogenic growth and blue- and far-red-light regulated gene expression. Activates multiple TIFY/JAZ promoters. Positive regulator of lateral root formation. Regulates sesquiterpene biosynthesis. Subjected to proteasome-dependent proteolysis. The presence of the destruction element (DE) involved in turnover is required for the function to regulate gene transcription.
Gene References into Functions
  1. the 2.7 A crystal structure of the MYC2 bHLH domain complexed with G-box DNA, showing a cis-tetrameric structure, is reported. PMID: 28514654
  2. allantoin can activate the MYC2-regulated jasmonic acid signaling pathway through abscissic acid production. PMID: 26931169
  3. MYC2 interacts with ANAC019 to co-regulate the expression of NYE1 during jasmonic acid-induced chlorophyll degradation. PMID: 26407000
  4. Under salt stress, JA almost did not render a positive effect on the jin1 plants. It is concluded that the protein JIN1/MYC2 is involved in control of protective systems under salt stress. PMID: 27266252
  5. MYC2 is targeted by PUB10 for degradation during jasmonic acid responses. PMID: 26163577
  6. MYC2 differentially regulates GATA-box conaining promoters during seedling development in Arabidopsis PMID: 23857363
  7. Together, these results reveal that phosphorylation-coupled turnover of MYC2 stimulates its transcription activity. PMID: 23593022
  8. MYC2 regulates RGL3 expression through a direct association with its promotor. PMID: 22892320
  9. During jasmonate signaling, MED25 physically associates with the basic helix-loop-helix transcription factor MYC2 in promoter regions of MYC2 target genes and exerts a positive effect on MYC2-regulated gene transcription. PMID: 22822206
  10. MYC2 interacts with RGA proteinsin regulating sesquiterpene synthase gene expression PMID: 22669881
  11. jasmonic acid predominantly promotes MYC2 protein accumulation in the morning and TIME FOR COFFEE represses accumulation of the MYC2 PMID: 22693280
  12. MYC2 acts as a negative regulator of light induced gene expression. PMID: 22424472
  13. MYC2-mediated repression of PLT expression is involved in jasmonate inhibition of primary root growth. PMID: 21954460
  14. The jasmonate-responsive activity of the jasmonate-responsive element from the ORCA3 promoter interacts in vitro and in vivo with the basic helix-loop-helix transcription factor AtMYC2. PMID: 21306988
  15. High- and medium-affinity binding sites are over-represented in promoters of MYC2- or ERF1 (ethylene-responsive transcription factor 1B)-regulated genes, and therefore they may represent new cis-regulatory elements. PMID: 21284757
  16. The role of jasmonate and salicylic acid signaling in susceptibility to P. syringae infection in A. thaliana mutants is reported. PMID: 16838791
  17. These results provide new insights into the function of MYC2 and the transcriptional coordination of the jasmonic acid signaling pathway. PMID: 17616737
  18. results pinpoint MYC2 as a potential regulator in priming for enhanced JA-responsive gene expression during rhizobacteria-mediated ISR PMID: 18657213

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Subcellular Location
Nucleus.
Tissue Specificity
Widely expressed in the whole plant with the highest expression in stem. Constitutively expressed in dark- and light-grown seedlings.
Database Links

KEGG: ath:AT1G32640

STRING: 3702.AT1G32640.1

UniGene: At.22648

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