Recombinant Arabidopsis thaliana Transcriptional activator DEMETER (DME), partial

Code CSB-YP839701DOA
MSDS
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Source Yeast
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Code CSB-EP839701DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP839701DOA
MSDS
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Source Baculovirus
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Code CSB-MP839701DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DME
Uniprot No.
Alternative Names
DME; At5g04560/At5g04570/At5g04580; T32M21.160/T32M21.170/T32M21.180Transcriptional activator DEMETER; EC 3.2.2.-; DNA glycosylase-related protein DME
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator involved in gene imprinting. Catalyzes the release of 5-methylcytosine (5-meC) from DNA by a glycosylase/lyase mechanism. Allows the expression of the maternal copy of the imprinted MEA gene before fertilization, possibly by antagonizing or suppressing DNA methylation on target promoter. Probably acts by nicking the MEA promoter. Required for stable reproducible patterns of floral and vegetative development.
Gene References into Functions
  1. DME and ROS1 have a significant 5-hydroxymethylcytosine (5hmC) excision activity in vitro, although the study detected no 5hmC in Arabidopsis, suggesting that it is very unlikely for plants to utilize 5hmC as a DNA demethylation intermediate. PMID: 24661881
  2. Histone H1 is involved in DME-mediated DNA methylation and gene regulation at imprinted loci. PMID: 22519754
  3. DME is active and demethylates similar genes and transposons in the genomes of the vegetative and central cells in the male and female gametophytes PMID: 21518889
  4. Analyses indicate that AtLIG1 acts downstream of DME. The removal of methylated cytosine residues by DME involves the creation of DNA single-strand breaks and our results suggest that AtLIG1 repairs these breaks. PMID: 20023162
  5. Demethylation by DEMETER is necessary for genomic imprinting and defines the genomic methylation profile of Arabidopsis genes. PMID: 17409185
  6. Gene ontology analysis identified three molecular functions enriched in the DME-inducible RNA list: DNA or RNA binding, kinase activity, and transcription factor activity. PMID: 17964287
  7. control DNA methylation in Arabidopsis thaliana.[review] PMID: 18536342

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Subcellular Location
Nucleus.
Protein Families
DNA glycosylase family, DEMETER subfamily
Tissue Specificity
Mainly expressed in immature flower buds, then decreases as the flower matures. Expressed in the ovule carpels, but not expressed in pollen stamens. Expressed in developing and mature ovules (stages 12-14), then strongly decreases after fertilization.
Database Links

KEGG: ath:AT5G04560

STRING: 3702.AT5G04560.2

UniGene: At.33104

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