Recombinant Arabidopsis thaliana Transcriptional adapter ADA2b (ADA2B)

Code CSB-YP858953DOA
MSDS
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Source Yeast
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Code CSB-EP858953DOA
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Source E.coli
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Code CSB-EP858953DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP858953DOA
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Source Baculovirus
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Code CSB-MP858953DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ADA2B
Uniprot No.
Alternative Names
ADA2B; PRZ1; At4g16420; dl4235c; Transcriptional adapter ADA2b; AtADA2b; Protein PROPORZ 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-487
Target Protein Sequence
MGRSRGNFQN FEDPTQRTRK KKNAANVENF ESTSLVPGAE GGGKYNCDYC QKDITGKIRI KCAVCPDFDL CIECMSVGAE ITPHKCDHPY RVMGNLTFPL ICPDWSADDE MLLLEGLEIY GLGNWAEVAE HVGTKSKEQC LEHYRNIYLN SPFFPLPDMS HVAGKNRKEL QAMAKGRIDD KKAEQNMKEE YPFSPPKVKV EDTQKESFVD RSFGGKKPVS TSVNNSLVEL SNYNQKREEF DPEYDNDAEQ LLAEMEFKEN DTPEEHELKL RVLRIYSKRL DERKRRKEFI IERNLLYPNP FEKDLSQEEK VQCRRLDVFM RFHSKEEHDE LLRNVVSEYR MVKRLKDLKE AQVAGCRSTA EAERYLGRKR KRENEEGMNR GKESGQFGQI AGEMGSRPPV QASSSYVNDL DLIGFTESQL LSESEKRLCS EVKLVPPVYL QMQQVMSHEI FKGNVTKKSD AYSLFKIDPT KVDRVYDMLV KKGIAQL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for the function of some acidic activation domains, which activate transcription from a distant site. The exact mechanism of action is not yet known. ADA2 stimulates the acetyltransferase activity of GCN5 on free histones or nucleosomes, probably by opening up the promoter region. Mediates auxin and cytokinin signals in the control of cell proliferation and might be involved in repression of a freezing tolerance pathway at warm temperature. Involved in the positive regulation of salt-induced gene expression by maintaining locus-specific acetylation of histones H4 and H3.
Gene References into Functions
  1. required, with GCN5, in leaves and trichomes to couple nuclear content with cell growth and morphogenesis PMID: 29846775
  2. ADA2b is required for the expression of genes involved in abiotic stress either by controlling H3 and H4 acetylation. PMID: 21897124
  3. The role of SGF29a and ADA2b in salt stress responses, was explored PMID: 21193996
  4. PRZ1 is involved in the modulation of histone modifications and corresponding adjustments in gene expression of KIP RELATED PROTEIN (KRP) CDK inhibitor genes in response to auxin. PMID: 20479223
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in roots, leaves, stems, flowers and siliques, with the strongest activity in the meristematic zones.
Database Links

KEGG: ath:AT4G16420

STRING: 3702.AT4G16420.1

UniGene: At.4412

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