Recombinant Arabidopsis thaliana Transcriptional corepressor LEUNIG (LUG), partial

Code CSB-YP861883DOA
MSDS
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Source Yeast
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Code CSB-EP861883DOA
MSDS
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Source E.coli
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Code CSB-EP861883DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP861883DOA
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Source Baculovirus
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Code CSB-MP861883DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LUG
Uniprot No.
Alternative Names
LUG; RON2; At4g32551; L23H3.30Transcriptional corepressor LEUNIG; Protein ROTUNDA2
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription repressor subunit of the SEU-LUG transcriptional corepressor of the C class floral homeotic gene AGAMOUS during the early stages of floral meristem development. Is part of the A class cadastral complex that define the boundaries between the A and C class homeotic genes expression and function. Interacts together with APETALA2 and SEUSS to repress AGAMOUS expression. Also plays a role in ovule and pollen development. Implicated in embryo and floral development. Involved in post-synthesis cell wall modifications necessary for mucilage extrusion from seeds upon imbibition, probably by promoting the expression of genes required for mucilage maturation. Involved in vegetative leaf morphogenesis at later stages of leaf development by restricting cell expansion during leaf growth. Regulates the maintenance on leaf polarity and meristem activity as well as the initiation of embryonic shoot apical meristem (SAM) development.
Gene References into Functions
  1. LEUNIG (LUG) and SEUSS (SEU) co-repressors repress miR172 expression in the outer whorls of Arabidopsis thaliana flowers. PMID: 21610026
  2. lug mutant seeds but not seu exhibited weak mucilage defects. PMID: 21362134
  3. SEUSS and LEUNIG control blade cell number and vasculature development within the petal and regulate petal polarity along the adaxial/abaxial axis. PMID: 16625397
  4. Functional diversification between LUH and LUG is evidenced by the inability of 35S::LUH overexpression to rescue lug mutants and by the opposite expression trends of LUG and LUH in response to biotic and abiotic stresses PMID: 18390806
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in young flower primordia, later becomes localized to petals, stamens and carpels. Is also expressed in vegetative tissues. Expressed in roots, stems, leaves, seedlings, apex, flowers, flower organs and seeds.
Database Links

KEGG: ath:AT4G32551

STRING: 3702.AT4G32551.2

UniGene: At.22027

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