Recombinant Arabidopsis thaliana Ubiquitin carboxyl-terminal hydrolase 13 (UBP13), partial

Code CSB-YP802935DOA
MSDS
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Source Yeast
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Code CSB-EP802935DOA
MSDS
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Source E.coli
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Code CSB-EP802935DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP802935DOA
MSDS
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Source Baculovirus
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Code CSB-MP802935DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
UBP13
Uniprot No.
Alternative Names
UBP13; At3g11910; F26K24.20; MEC18.1; Ubiquitin carboxyl-terminal hydrolase 13; EC 3.4.19.12; Deubiquitinating enzyme 13; AtUBP13; Ubiquitin thioesterase 13; Ubiquitin-specific-processing protease 13
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Positive regulator of root meristem development that, together with UBP12, prevents the ubiquitination and turnover of RGFR1 induced by the RGF1 hormone peptide, thus influencing PLT1 and PLT2 expression.
Gene References into Functions
  1. UBP12/13 are regulators of the RGF1-RGFR1-PLT1/2 signaling pathway and UBP12/13 can counteract RGF1-induced RGFR1 ubiquitination, stabilize RGFR1, and maintain root cell sensitivity to RGF1 PMID: 29339500
  2. UBP12 and UBP13 belong to an ancient gene family and represent plant homologues of metazoan USP7. Drosophila USP7 shares a function in heterochromatic gene repression with UBP12/13 and their homologue UBP26. In summary, USP7-like proteins are essential for gene silencing in diverse genomic contexts. PMID: 27525512
  3. The Deubiquitinating Enzymes UBP12 and UBP13 Positively Regulate MYC2 Levels in Jasmonate Responses PMID: 28536144
  4. UBP12 and UBP13 proteins play important role in the control of the circadian clock and photoperiodic flowering. PMID: 23645632
  5. AtUBP13 is a deubiquitinating enzyme capable of cleaving lysine-48-linked ubiquitin chains. PMID: 21388379

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Protein Families
Peptidase C19 family
Database Links

KEGG: ath:AT3G11910

STRING: 3702.AT3G11910.1

UniGene: At.25337

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