Recombinant Arabidopsis thaliana WD-40 repeat-containing protein MSI1 (MSI1)

Code CSB-YP522782DOA
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP522782DOA
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP522782DOA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP522782DOA
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP522782DOA
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
MSI1
Uniprot No.
Alternative Names
MSI1; At5g58230; MCK7.10; Histone-binding protein MSI1; CAF-1 p48 homolog; Chromatin assembly factor 1 subunit MSI1; CAF-1 subunit MSI1; Protein MULTICOPY SUPPRESSOR OF IRA 1; AtMSI1; Protein medicis; WD-40 repeat-containing protein MSI1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-424
Target Protein Sequence
MGKDEEEMRG EIEERLINEE YKIWKKNTPF LYDLVITHAL EWPSLTVEWL PDREEPSGKD YSVQKMILGT HTSESEPNYL MLAQVQLPLD DTESEARQYD DDRSEFGGFG CATGKVQIIQ QINHDGEVNR ARYMPQNPFI IATKTVNAEV YVFDYSKHPS KPPLDGACNP DLKLRGHSSE GYGLSWSKFK QGHLLSGSDD AQICLWDINA TPKNKSLDAQ QIFKAHEGVV EDVAWHLRHE YLFGSVGDDQ YLLIWDLRSP SASKPVQSVV AHSMEVNCLA FNPFNEWVVA TGSTDKTVKL FDLRKLSTAL HTFDSHKEEV FQVGWNPKNE TILASCCLGR RLMVWDLSRI DEEQTVEDAE DGPPELLFIH GGHTSKISDF SWNPCEDWVI SSVAEDNILQ IWQMAENIYH DEDDAPGEEP SKAS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair, and the fertilization independent seed (FIS) complex, a polycomb group protein complex which is required to maintain the transcriptionally repressive state of homeotic genes throughout development. Required for several aspects of plant development including normal leaf expansion, correct development of flowers, normal endosperm development, repression of parthenogenetic seed development and repression of floral homeotic genes in leaf tissue. Also required for the recruitment of chromosomal DNA into heterochromatic chromocenters. Also involved in regulation of imprinted genes. Acts together with RBR1 to repress the expression of MET1 during female gametogenesis. This in turn activates expression of the imprinted genes FIS2 and FWA.
Gene References into Functions
  1. MSI1 is a novel gene which regulates flowering. PMID: 16554362
  2. These results reveal that the functions of FAS1, FAS2 and MSI1 are not restricted to meristems, but are also needed to control genome replication at multiple steps of development. PMID: 17021044
  3. Data suggest that the Polycomb group protein MSI1 functions in the female gametophyte to determine seed development in plants. PMID: 17855429
  4. Rb-dependent activation is mediated by direct transcriptional repression of MET1 during female gametogenesis. PMID: 18700816
Subcellular Location
Nucleus.
Protein Families
WD repeat RBAP46/RBAP48/MSI1 family
Tissue Specificity
Ubiquitously expressed. Strongly expressed in floral buds and flowers, the female gametophyte and the sporophytic tissue of the ovules. Also strongly expressed in developing embryos following fertilization.
Database Links

KEGG: ath:AT5G58230

STRING: 3702.AT5G58230.1

UniGene: At.49185

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2025 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1