Recombinant Beta-catenin/armadillo-related protein 1 (bar-1), partial

Code CSB-YP623232CXY
MSDS
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Source Yeast
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Code CSB-EP623232CXY
MSDS
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Source E.coli
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Code CSB-EP623232CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP623232CXY
MSDS
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Source Baculovirus
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Code CSB-MP623232CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
bar-1
Uniprot No.
Alternative Names
bar-1; pvl-1; spy-1; C54D1.6Beta-catenin/armadillo-related protein 1; Protruding vulva protein 1; Suppressor of polyray 1
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Participates in the Wnt signaling pathway which affects cell fate and may regulate the stem cell divisions of seam cells during larval development. Functions as a transcriptional activator but is dependent on the interaction with pop-1. Involved in maintaining lin-39 Hox expression and regulating glr-1 abundance at the synapses. Required for mab-5 expression during Q neuroblast migration and for oxidative stress-induced daf-16 signaling. Has roles in egg laying, vulva precursor cell fate determination, Q neuroblast migration, posterior ectodermal cell P12 specification, movement, body length, male tail development and dauer induction. Functionally redundant to wrm-1 and hmp-2.
Gene References into Functions
  1. Neuroprotection from mutant huntingtin toxicity requires FOXO partner bar-1/Beta-catenin. PMID: 22956852
  2. promoter elements necessary for bar-1 expression in several cell types, including a 321-bp element sufficient for expression in ventral cord neurons and a 1.1-kb element sufficient for expression in the developing vulva and adult seam cells PMID: 15282167
  3. Caenorhabditis elegans, both WRM-1/beta-catenin and LIT-1 kinase localize to the anterior cell cortex during asymmetric cell division but to the nucleus of the posterior daughter afterward PMID: 16077003
  4. These results suggest that beta-catenin and APC may regulate asymmetric divisions or Wnt signaling. PMID: 17276345
  5. Membrane trafficking regulated by ipla-1 provides a mechanism to control the cortical asymmetry of beta-catenin. PMID: 18497747
  6. NHR-25 cooperates with the Wnt/beta-catenin asymmetry pathway to control differentiation of the T seam cell. PMID: 19654209

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Subcellular Location
Cytoplasm. Nucleus. Membrane. Cell junction.
Protein Families
Beta-catenin family
Tissue Specificity
Expressed in body wall muscle cells, hypodermal seam cells, gonad sheath cells and vulval precursor cells.
Database Links

KEGG: cel:CELE_C54D1.6

STRING: 6239.C54D1.6

UniGene: Cel.5480

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