Recombinant Bovine Insulin-like 3(INSL3)

Code CSB-YP011747BO
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Source Yeast
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Code CSB-EP011747BO
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Source E.coli
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Code CSB-EP011747BO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011747BO
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Source Baculovirus
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Code CSB-MP011747BO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names INSL3
Uniprot No. O77801
Alternative Names INSL3; RLFInsulin-like 3; Leydig insulin-like peptide; Ley-I-L; Relaxin-like factor) [Cleaved into: Insulin-like 3 B' chain; Insulin-like 3 B chain; Insulin-like 3 A chain]
Species Bos taurus (Bovine)
Expression Region 22-66
Protein Length Cytoplasmic domain
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor.
Gene References into Functions
  1. These results suggest that reduced secretions of IGF-I, INSL3, and inhibin surrounding puberty may be associated with semen aberration in beef bulls. PMID: 29597124
  2. These results suggested an acute regulation of INSL3 by luteinizing hormone (LH) because INSL3 concentrations increased immediately after endogenous and exogenous LH stimulation. PMID: 26318230
  3. INSL3 plays a luteotropic role as a local regulator in the bovine corpus luteum. PMID: 23220928
  4. BMP6 has a role in downregulating INSL3 and CYP17A1 and other proteins that modulate ovarian androgen production PMID: 23530236
  5. The likely role of INSL3 as an important intrafollicular modulator of thecal interna cell function/steroidogenesis. PMID: 23546605
  6. INSL3 provides the first example of a gender-specific fetal hormone with the potential to influence both placental and maternal physiology PMID: 21603619
  7. relaxin-like factor mRNA was highly expressed in the corpus luteum on days 7 and 14 of pregnancy PMID: 15956752

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Subcellular Location Secreted.
Protein Families Insulin family
Tissue Specificity Expressed exclusively in Leydig cells of the testis.
Database Links

KEGG: bta:281870

STRING: 9913.ENSBTAP00000027848

UniGene: Bt.91145

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
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