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Recombinant Bovine Insulin-like 3 (INSL3), partial

Recombinant Bovine Insulin-like 3 (INSL3), partial

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Code	CSB-EP011747BO
Abbreviation	Recombinant Bovine INSL3 protein, partial
MSDS	MSDS Datasheet
Size	$256
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

INSL3

Uniprot No.

O77801

Research Area

Others

Species

Bos taurus (Bovine)

Source

E.coli

Expression Region

22-66aa

Target Protein Sequence

GPAAAQEAPEKLCGHHFVRALVRLCGGPRWSSEEDGRPVAGGDRE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

20.1 kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-KSI-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Bovine INSL3 protein is produced by manipulating the expression of specific genes in e.coli cells. A DNA sequence encoding the Bovine INSL3 protein (22-66aa) is fused to an expression vector and subsequently transformed into e.coli cells. The cells that successfully integrate the vector are selected and cultured to induce the expression of the desired protein. A N-terminal 6xHis-KSI tag is attached to the protein. The recombinant Bovine INSL3 protein protein is then purified using affinity purification, achieving a purity level of over 90%, as confirmed by SDS-PAGE analysis.

Customer Reviews and Q&A

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Target Background

Function

Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor.

Gene References into Functions

These results suggest that reduced secretions of IGF-I, INSL3, and inhibin surrounding puberty may be associated with semen aberration in beef bulls. PMID: 29597124
These results suggested an acute regulation of INSL3 by luteinizing hormone (LH) because INSL3 concentrations increased immediately after endogenous and exogenous LH stimulation. PMID: 26318230
INSL3 plays a luteotropic role as a local regulator in the bovine corpus luteum. PMID: 23220928
BMP6 has a role in downregulating INSL3 and CYP17A1 and other proteins that modulate ovarian androgen production PMID: 23530236
The likely role of INSL3 as an important intrafollicular modulator of thecal interna cell function/steroidogenesis. PMID: 23546605
INSL3 provides the first example of a gender-specific fetal hormone with the potential to influence both placental and maternal physiology PMID: 21603619
relaxin-like factor mRNA was highly expressed in the corpus luteum on days 7 and 14 of pregnancy PMID: 15956752