Recombinant Bovine Perilipin-5 (PLIN5)

Code CSB-YP407507BO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP407507BO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP407507BO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP407507BO
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PLIN5
Uniprot No.
Alternative Names
PLIN5; LSDP5; Perilipin-5; Lipid storage droplet protein 5
Species
Bos taurus (Bovine)
Expression Region
1-456
Target Protein Sequence
MSEDEAAQAP GPSLWEQDQQ SVVQRVAALP LIRATCSAVS EAYSAAKDRH PLLGSACRLA EHCVCDLTTR ALDHAQPLLS HLQPQLATVN DLACRGLDKL EEKLPFLQQP SETVVTSAKG VVASGVTGMV GLARQGRRWS VELKRSVSHA MDVVLEKSEE LVDHFLPMTE EELAALAAEA EGPEVGSVEE QRKHQGYFVR LGSLSTRLRH LAYEHSLGKL RQKKHHAQDM LAQLQETLEL IHRMQCGVTP ITPARPGKVH ELWEDWSQRP LENGRRRHSQ AELETLVLSR GLMEELQSTV DALETSVRGL PPSAQEKVAE VRRSVDALQA AFADARRFGD VPAAVLAEGR GSMARAHACV DELLELVVQA VPLPWLVGPF APILVERPEP PPDLEALVDE VVGGPDPRWA HLDWPAQQRA WQAQHGEGTV LSGNIPEEEP EPPSRPKHTL MPELDF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Lipid droplet-associated protein that maintains the balance between lipogenesis and lipolysis and also regulates fatty acid oxidation in oxidative tissues. Recruits mitochondria to the surface of lipid droplets and is involved in lipid droplet homeostasis by regulating both the storage of fatty acids in the form of triglycerides and the release of fatty acids for mitochondrial fatty acid oxidation. In lipid droplet triacylglycerol hydrolysis, plays a role as a scaffolding protein for three major key lipolytic players: ABHD5, PNPLA2 and LIPE. Reduces the triacylglycerol hydrolase activity of PNPLA2 by recruiting and sequestering PNPLA2 to lipid droplets. Phosphorylation by PKA enables lipolysis probably by promoting release of ABHD5 from the perilipin scaffold and by facilitating interaction of ABHD5 with PNPLA2. Also increases lipolysis through interaction with LIPE and upon PKA-mediated phosphorylation of LIPE.
Subcellular Location
Lipid droplet. Cytoplasm. Mitochondrion.
Protein Families
Perilipin family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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