Recombinant Bovine Phosphotriesterase-related protein (PTER)

To generate the recombinant bovine PTER protein, the gene fragment coding for the full-length bovine PTER (1-349aa) is cloned into an expression vector designed for E. coli expression. The expression vector is carefully constructed to include an N-terminal 10xHis-tag and a C-terminal Myc-tag, enabling purification and detection of the recombinant protein. Subsequently, the recombinant expression vector carrying the PTER gene fragment is introduced into competent E. coli cells. Transformed cells are selected through antibiotic resistance on agar plates specific to the expression vector's selection marker. The selected E. coli cells are then cultivated under suitable conditions, allowing for optimal protein expression. Protein expression is induced using an appropriate inducer, such as IPTG. Following induction, the E. coli cells are harvested by centrifugation and cell lysis is performed to release the cellular contents, including the expressed recombinant PTER protein. The purity of the recombinant PTER protein is assessed using SDS-PAGE analysis, confirming a purity level exceeding 90%.