Recombinant Bovine Tracheal antimicrobial peptide

Code CSB-YP328513BO
MSDS
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Source Yeast
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Code CSB-EP328513BO
MSDS
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Source E.coli
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Code CSB-EP328513BO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP328513BO
MSDS
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Source Baculovirus
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Code CSB-MP328513BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Uniprot No.
Alternative Names
; Tracheal antimicrobial peptide; TAP
Species
Bos taurus (Bovine)
Expression Region
27-64
Target Protein Sequence
NPVS CVRNKGICVP IRCPGSMKQI GTCVGRAVKC CRKK
Protein Length
Cytoplasmic domain
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Has antibacterial activity in vitro against Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, and Pseudomonas aeruginosa. In addition, the peptide is active against Candida albicans, indicating a broad spectrum of activity.
Gene References into Functions
  1. Sequencing of exon 2 of the TAP gene from 23 cattle revealed a prevalent non-synonymous single nucleotide polymorphism (SNP) A137G, encoding either serine or asparagine at residue 20 of the mature peptide. PMID: 23333196
  2. Twenty four hours post E. coli stimulation, IL1b, IL6, IL8, TNF and LAP gene expression was decreased compared to 6h, whereas TAP and SAA3 expression was further increased to 209- and 3452-fold (P<0.05). PMID: 23200820
  3. The results from this study clearly demonstrate the involvement of octamer transcription factor-1 in the regulation of tracheal antimicrobial peptide and lingual antimicrobial peptide expression. PMID: 21674298
  4. The production of tracheal antimicrobial peptide in mammary epithelial cells in reponse to bacteria, drugs, and prolactins is reported. PMID: 19181355
Subcellular Location
Secreted.
Protein Families
Beta-defensin family, LAP/TAP subfamily
Tissue Specificity
Tracheal epithelium.
Database Links

KEGG: bta:286837

STRING: 9913.ENSBTAP00000056499

UniGene: Bt.510

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