Recombinant Caenorhabditis elegans Calreticulin (crt-1)

Code CSB-YP004458CXY
MSDS
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Source Yeast
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Code CSB-EP004458CXY
MSDS
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Source E.coli
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Code CSB-EP004458CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP004458CXY
MSDS
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Source Baculovirus
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Code CSB-MP004458CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
crt-1
Uniprot No.
Alternative Names
crt-1; Y38A10A.5Calreticulin
Species
Caenorhabditis elegans
Expression Region
16-395
Target Protein Sequence
EVYFK EEFNDASWEK RWVQSKHKDD FGAFKLSAGK FFDVESRDQG IQTSQDAKFY SRAAKFDKDF SNKGKTLVIQ YTVKHEQGID CGGGYVKVMR ADADLGDFHG ETPYNVMFGP DICGPTRRVH VILNYKGENK LIKKEITCKS DELTHLYTLI LNSDNTYEVK IDGESAQTGS LEEDWDLLPA KKIKDPDAKK PEDWDEREYI DDAEDAKPED WEKPEHIPDP DAKKPEDWDD EMDGEWEPPM IDNPEYKGEW KPKQIKNPAY KGKWIHPEIE NPEYTPDDEL YSYESWGAIG FDLWQVKSGT IFDNIIITDS VEEAEAHAAE TFDKLKTVEK EKKEKADEET RKAEEEARKK AEEEKEAKKD DDEEEKEEEE GHDEL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Probably by controlling the folding of extracellular matrix protein unc-52/Perlecan, may play a role in the formation of fibrous organelles, a hemidesmosome-like structure attaching muscles to the epidermis. Protects dopaminergic neurons against oxidative stress-induced neurodegeneration.
Gene References into Functions
  1. Data show that the neuroligin-like GLIT-1, the canonical apoptosis pathway and the calreticulin CRT-1 are required to prevent 6-OHDA-induced dopaminergic neurodegeneration. PMID: 29346364
  2. CRT-1 controls by as little as 2-fold the abundance of UNC-52/perlecan, an essential hemidesmosome extracellular matrix ligand. PMID: 20153198
  3. Tunicamycin-induced UPR pathway culminates in the up-regulation of crt-1, which protects the worm from deleterious accumulation of unfolded proteins in the ER PMID: 17651753
Subcellular Location
Endoplasmic reticulum lumen.
Protein Families
Calreticulin family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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