Recombinant Caenorhabditis elegans Protein glp-1 (glp-1), partial

Code CSB-YP319558CXY
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP319558CXY
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP319558CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP319558CXY
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP319558CXY
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
glp-1
Uniprot No.
Alternative Names
glp-1; emb-33; F02A9.6Protein glp-1
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Essential signaling protein which has a major role in germline and embryonic development; involved in cell fate decisions that require cell-cell interactions. Probable membrane-bound receptor for putative ligands lag-2 and apx-1. Upon ligand activation, and releasing from the cell membrane, the glp-1/Notch intracellular domain (NICD) probably forms a transcriptional activator complex with lag-1 and lag-3 and regulates expression of various genes; targets in the germline include lst-1 and sygl-1. Involved in the specification of the cell fates of the blastomeres, ABa and ABp. Proper signaling by glp-1 induces ABa descendants to produce anterior pharyngeal cells, and ABp descendants to adopt a different fate. Contributes to the establishment of the dorsal-ventral axis in early embryos. Required in postmitotic neurons in order to maintain the developmentally arrested larval state known as dauer, probably in response to lag-2. Regulates germ cell mitotic proliferation probably by regulating MAP kinase phosphatase lip-1 expression. Required for oocyte growth control. Plays a negative role in lifespan.
Gene References into Functions
  1. These results provide the first experimental evidence of an essential role for HSP90 in Notch signaling in development. PMID: 29507057
  2. The authors find that Notch-dependent active transcription sites occur in a probabilistic fashion and, unexpectedly, do so in a steep gradient across the stem cell pool. PMID: 27705743
  3. Zygotically expressed MOM-2 (Wnt) is responsible for two distinct cell-cell interactions as the MS blastomere produces one-third of the body wall muscles. Both the inhibitory and the activating interactions require precise spatiotemporal expression of zygotic MOM-2, which is dependent upon two distinct GLP-1 Notch signals. PMID: 28049659
  4. Study suggests that GLP-1 signaling is directly responsible for driving all stages of proliferation in the wild-type germline, and loss of its activity causes differentiation without intervening, distinctly regulated transit-amplifying divisions. PMID: 26158953
  5. glp-1(ar202), a temperature-sensitive gain-of-function C. elegans NOTCH mutant, was employed to delineate NOTCH-driven tumor responses to radiotherapy. PMID: 26120834
  6. regulatory element is highly conserved in the paralogous glp-1 genomic environment, suggesting redundant developmental and physiological roles for the two Notch paralogs in the C. elegans nervous system. PMID: 23305862
  7. POS-1 regulates glp-1 mRNA translation by blocking access of other RNA-binding proteins to a key regulatory sequence PMID: 23034181
  8. The functional defect in glp-1(Pro) mutants occurs prior to or at the level of ligand interaction. PMID: 12586701
  9. the balance between POS-1 and SPN-4 controls the translation of maternal glp-1 mRNA PMID: 12702662
  10. Results suggest that TBX-37 and TBX-38 play a key role in distinguishing the outcomes of two sequential Glp-1/Notch-mediated interactions. PMID: 15056620
  11. Data support a model in which GLP-1 function in MSP-dependent oocyte growth is separable from its role in the proliferation versus meiotic entry decision. PMID: 19502484

Show More

Hide All

Subcellular Location
Cell membrane; Single-pass type I membrane protein. Cell projection, axon.; [glp-1/Notch intracellular domain]: Nucleus.
Tissue Specificity
Expressed in the distal mitotic region of the germ line. May be absent from the gonadal distal tip cell (DTC).
Database Links

KEGG: cel:CELE_F02A9.6

STRING: 6239.F02A9.6

UniGene: Cel.6196

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1