Recombinant Chicken Tropomyosin alpha-1 chain (TPM1)

Code CSB-YP024104CH
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP024104CH
MSDS
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Source E.coli
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Code CSB-EP024104CH-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP024104CH
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP024104CH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TPM1
Uniprot No.
Alternative Names
TPM1; Tropomyosin alpha-1 chain; Alpha-tropomyosin; Tropomyosin-1
Species
Gallus gallus (Chicken)
Expression Region
1-284
Target Protein Sequence
MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEERSKQLE DELVALQKKL KGTEDELDKY SESLKDAQEK LELADKKATD AESEVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA DESERGMKVI ENRAQKDEEK MEIQEIQLKE AKHIAEEADR KYEEVARKLV IIEGDLERAE ERAELSESKC AELEEELKTV TNNLKSLEAQ AEKYSQKEDK YEEEIKVLTD KLKEAETRAE FAERSVTKLE KSIDDLEDEL YAQKLKYKAI SEELDHALND MTSI
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Gene References into Functions
  1. Structural analysis of smooth muscle tropomyosin alpha and beta isoforms. PMID: 22119916
  2. The tendency of smooth muscle tropomyosin to form semi-rigid polymers with continuous and undampened rigidity may compensate for the lack of troponin-based structural support in smooth muscles. PMID: 20682264
  3. An interaction between CHASM and tropomyosin, is demonstrated. PMID: 20627103
  4. steady state actomyosin ATPase is inhibited by caldesmon and tropomyosin PMID: 16540476
  5. All smooth muscle tropomyosins regulate binding to actin Tm in the same way. PMID: 17042503
  6. role, in addition to its cooperative control of myosin function, establishes tropomyosin as a universal regulator of the multifaceted actin cytoskeleton PMID: 17569543
  7. The results show that residues K5, K7, and D280 are essential to the stability of the alpha-tropomyosin complex. PMID: 18536019
  8. The Mg(2+)-induced increase in stability of the C-terminal half of Tm is sensitive to mutations near the C-terminus. PMID: 19280641

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Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Tropomyosin family
Database Links

KEGG: gga:396366

UniGene: Gga.4108

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