Recombinant Cyclin-dependent kinase 1 (cdk-1)

Code CSB-YP333911CXY
MSDS
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Source Yeast
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Code CSB-EP333911CXY
MSDS
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Source E.coli
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Code CSB-EP333911CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP333911CXY
MSDS
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Source Baculovirus
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Code CSB-MP333911CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
cdk-1
Uniprot No.
Alternative Names
cdk-1; ncc-1; T05G5.3; Cyclin-dependent kinase 1; CDK1; EC 2.7.11.22; EC 2.7.11.23; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase
Species
Caenorhabditis elegans
Expression Region
1-332
Target Protein Sequence
MDPIREGEVA HEGDSVYTLN DFTKLEKIGE GTYGVVYKGK NRRTNAMVAM KKIRLESEDE GVPSTAVREI SLLKELQHPN VVGLEAVIMQ ENRLFLIFEF LSFDLKRYMD QLGKDEYLPL ETLKSYTFQI LQAMCFCHQR RVIHRDLKPQ NLLVDNNGAI KLADFGLARA IGIPIRVYTH EVVTLWYRAP EILMGAQRYS MGVDMWSIGC IFAEMATKKP LFQGDSEIDE LFRIFRVLGT PTELEWNGVE SLPDYKATFP KWRENFLRDK FYDKKTGKHL LDDTAFSLLE GLLIYDPSLR LNAKKALVHP YFDNMDTSKL PAGNYRGELE LF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
Gene References into Functions
  1. CDK-1 regulates PLK-1 activity during mitosis in C. elegans embryos through multisite phosphorylation of the PLK-1 activator SPAT-1 PMID: 26038951
  2. CDK-1 blocks rotation by inhibiting dynein association with microtubules. PMID: 21690306
  3. Use of loss- and gain-of-function genetic approaches demonstrates that CYY-1, a cyclin box-containing protein, drives synapse removal in this process. PMID: 21609829
  4. NPP-16 and CDK-1 function to arrest prophase blastomeres in C. elegans embryos PMID: 20053678
  5. Co-depletion studies of CDK-1 and WEE-1.3 demonstrate that WEE-1.3 is dispensable in the absence of CDK-1, suggesting that CDK-1 is a major target of WEE-1.3 in C. elegans oocytes. PMID: 16421191
  6. MBK-2 is activated during oocyte maturation by CDK-1-dependent phosphorylation of serine 68, a residue outside of the kinase domain required for full activity in vivo. PMID: 19879842

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Subcellular Location
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
Database Links

KEGG: cel:CELE_T05G5.3

STRING: 6239.T05G5.3.2

UniGene: Cel.23379

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