Recombinant DAZ protein 1 (daz-1)

Code CSB-YP631333CXY
MSDS
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Source Yeast
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Code CSB-EP631333CXY
MSDS
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Source E.coli
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Code CSB-EP631333CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP631333CXY
MSDS
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Source Baculovirus
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Code CSB-MP631333CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
daz-1
Uniprot No.
Alternative Names
daz-1; F56D1.7DAZ protein 1
Species
Caenorhabditis elegans
Expression Region
1-499
Target Protein Sequence
MMSPPLRYQK DQQNQQHQQN QSQQAAHQMV SFMPQTPTSL PSTPIQLYPT GAAALIPAPP TYELIPNRIF VGGFPTSTTE TELREHFEKF FAVKDVKMVK SLDGQSKGYG FITFETEDQA EEIRKLTPKQ LEFRSRKLNL GPAIRKINSN SFQPSYAIAT PSQLVAASPG PFSYAIPASP SPYSGYSYPA SPQMFVYPPL RSQDQSRQQS EQQTTPQNSP TNLQHQQSPQ VFFGGDQDPI RSYASAVAGV EKSEVSPEKH ESVSPQPLLP NQNVLNTQYS QGQQQWNSNV QQQQQQQMDS NNGGPYYNEN YSQGYTRPHP YQQFAQSGVY MNSQGMYHSS YSYMTPPLAP GQYPQMMSAP YWQQQQQHHQ SHAYAGYNPA YNNWVGPSGD MNQFKQNPSN YFYQNYPGNF SQQHTMGNNE NTFSLPLQAP RQGKKSRKPS ECQDKKTKSP IKGARTERPS SSASTPDAKY QKNHRYPVHL SPLSASLQSL AISSPTKNN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
RNA-binding protein that plays a central role in oogenesis, but not for spermatogenesis. Required for meiotic entry and germline differentiation, at the pachytene stage of meiosis I of female germline regardless of the sex of the soma. May act by regulating translation of specific mRNAs, possibly by binding to their 3'-UTR.
Gene References into Functions
  1. DAZ-1 in C. elegans plays essential roles in female premeiotic and early meiotic germ cells, probably via regulating the translational activity of specific target genes required for the progression of oogenesis. PMID: 15572146
  2. daz-1 plays a role upstream of the pathway for germ cell sex determination. PMID: 16641369
  3. in a two hybrid assay,daz- as bait interacted with cpb-3 PMID: 16678151
  4. all germline samples studied were found to be free from AZFc microdeletions and CNP of the DAZ genes. PMID: 17308897
Protein Families
RRM DAZ family
Tissue Specificity
Germline specific. More strongly expressed during oogenesis than during spermatogenesis. During the larval stages, it is more abundant at the distal region than the proximal region of the gonad. In young adult hermaphrodites, it is expressed at a very low
Database Links

KEGG: cel:CELE_F56D1.7

STRING: 6239.F56D1.7

UniGene: Cel.6835

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