Recombinant Monkeypox virus DNA-directed RNA polymerase 132 kDa polypeptide (RPO132), partial

Code CSB-YP851803MHV
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Source Yeast
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Code CSB-EP851803MHV
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Source E.coli
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Code CSB-EP851803MHV-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP851803MHV
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Source Baculovirus
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Code CSB-MP851803MHV
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RPO132
Uniprot No.
Alternative Names
RPO132; A25R; DNA-directed RNA polymerase 132 kDa polypeptide; EC 2.7.7.6
Species
Monkeypox virus
Target Protein Sequence
MKKNTVSEMD QRLGYKFLVP DPKAGVFYRP LHFQYVSYSN FILHRLHEIL TVKRPLLSFK NNTERIMIEI SNVKVTPPDY SPIIASIKGK SYDALATFTV NIFKEVMTKE GISITKISSY EGKDSHLIKI PLLIGYGNKN PLDTAKYLVP NVIGGVFINK QSVEKVGINL VEKITTWPKF RVVKPNSFTF SFSSVSPPNV LPTRYRHYKI SLDISQLEAS NISSTKTFIT VNIVLLSQYL SRVSLEFIRR SLSYDMPPEV VYLVNAIIDS AKRLTESITD FDIDTYINDL VEAEHIKQKS QLTINEFKYE MLHNFLPHMN YTPDQLKGFY MISLLRKFLY CIYHTSRYPD RDSMVCHRIL TYGKYFETLA HDELENYIGN IRNDIMNNHK NRGTYAVNIH VLTTPGLNHA FSSLLSGKFK KSDGSYRTHP HYSWMQNISI PRSVGFYPDQ VKISKMFSVR KYHPSQYLYF CSSDVPERGP QVGLVSQLSV LSSITNILTS EYLDLEKKIC EYIRSYYKDD ISYFETGFPI TIENALVASL NPNMICDFVT DFRRRKRMGF FGNLEVGITL VRDHMNEIRI NIGAGRLVRP FLVVDNGELM TDVCPELESR LDDMTFSDIQ KEFPHVIEMV DIDQFTFSNV CESVQKFRMM SKDERKQYDL CDFPAEFRDG YVASSLVGIN HNSGPRAILG CAQAKQAISC LSSDIRNKID NGIHLMYPER PIVISKALET SKIAANCFGQ HVTIALMSYK GINQEDGIII KKQFIQRGGL DIVTAKKHQV EIPLENFNNK ERDRSNAYSK LESNGLVRLN AFLESGDAMA RNISSRTLED DFARDNQISF DVSEKYTDMY KSRVERVQVE LTDKVKVRVL TMKERRPILG DKFTTRTSQK GTVAYIADET ELPYDENGIT PDVIINSTSI FSRKTISMLI EVILTAAYST KPYNNKGENR PVCFPSSNET SIDAYMQFAK QCYEYSNPKL SEEELSDKIF CEKILYDPET DKPYESKVFF GPIYYLRLRH LTQDKATVRC RGKKTKLIRQ ANEGRKRGGG IKFGEMERDC LIAHGAANTI TEVLKDSEED YQDVYICENC GDIAAQIKSI NTCLRCSKLN LSPLLTKIDT THVSKVFLTQ MNARGVKVKL DFERRPPSFY KPLDKVDLKP SFLV
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Part of the DNA-dependent RNA polymerase which catalyzes the transcription of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of early, intermediate and late genes. DNA-dependent RNA polymerase associates with the early transcription factor (ETF), itself composed of D6 and A7, thereby allowing the early genes transcription. Late transcription, and probably also intermediate transcription, require newly synthesized RNA polymerase.
Subcellular Location
Virion.
Protein Families
RNA polymerase beta chain family
Database Links

KEGG: vg:928976

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