Recombinant DT3C protein

Code CSB-EP360556CQR1
Size How to order?
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Description

The product CSB-EP360556CQR1 is a recombinant DT3C protein. The Gly33-Lys417 encoding the Corynephage beta diphtheria toxin that was linked to the Ile291-Glu497 encoding the Streptococcus sp. group G spg and was fused with an N-terminal 6xHis-tag was expressed in the E.coli. The purity of this recombinant DT3C protein is greater than 90% measured by SDS-PAGE.

DT3C protein consists of a diphtheria toxin (DT) without a receptor binding domain and C1, C2, and C3 (3C) domains of Streptococcus protein. It is mainly used to detect the internalization efficiency of cancer cells to mAb in vitro and then to screen out more effective monoclonal antibodies. DT3C protein can mimic ADC drug function in vitro, and the preparation of the mAb-DT3C complex is relatively simple. DT3C protein can bind to any IgG from various species, such as human, mouse, rabbit, etc., to form mAb-DT3C conjugation, thus further expanding the scope of DT3C as a detection tool for mAb antibody internalization. Compared with Mab-ZAP, the mAb-DT3C conjugate is more stable, with a smaller molecular weight than mAb-Mab-ZAP. And the relevant experimental data show that the internalization efficiency of mAb-DT3C is higher than mAb-Mab-ZAP. According to incomplete statistics, DT3C as a tool to detect the internalization ability of mAb has been used in nearly 130 ADC drug mAb screening patents.

Purity Greater than 90% as determined by SDS-PAGE.
Target Names DT3C
Uniprot No. P00588 P19909
Research Area Others
Molecular Characterization
Species Corynephage beta
Source E.coli
Expression Region 33-417aa(P00588) & 291-497aa(P19909)
Target Protein Sequence GADDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDDDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELGLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEINFETRGKRGQDAMYEYMAQACAGNRVRRSVGSSLSCINLDWDVIRDKTKTKIESLKEHGPIKNKMSESPNKTVSEEKAKQYLEEFHQTALEHPELSELKTVTGTNPVFAGANYAAWAVNVAQVIDSETADNLEKTTAALSILPGIGSVMGIADGAVHHNTEEIVAQSIALSSLMVAQAIPLVGELVDIGFAAYNFVESIINLFQVVHNSYNRPAYSPGHKGGGGSGGGGSGGGGSIDEILAALPKTDTYKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQYANDNGVDGVWTYDDATKTFTVTE
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 69.4 kDa
Protein Length Linked Heterodimer
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.

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