Recombinant Danio rerio Dead end protein 1 (dnd)

Code CSB-YP742472DIL
MSDS
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Source Yeast
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Code CSB-EP742472DIL
MSDS
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Source E.coli
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Code CSB-EP742472DIL-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP742472DIL
MSDS
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Source Baculovirus
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Code CSB-MP742472DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
dnd
Uniprot No.
Alternative Names
dndDead end protein 1
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-411
Target Protein Sequence
MVGDMDAQQQ ELQQILNPQK LKSLQEWMQR NSITLTQVNG QRKYGGPPPG WQGPAPGSGC EVFISQIPND VYEDRLIPLF QSIGTIYEFR LMMNFSGQTR GFAYAKYGDP LTASAAVTTL HQYRLPEGGC LTVRRSTEKR QLRLGDLPVS MNESKLLMVL QMLSDGVEDV LLKPPGPKGK EVVALVNYTS HYAASMAKKV LVEAFRNRYG ISITVRWTSF SKSKRVEDTP QEDSCVTPLV LKPLSKPSLL HYDVPAHQSL LPLFRAVGGP TTSEQRDEMI PQPTIMSRNE LIPQSSIRQR DEMVPQLPIR PRDGMAPQSP ISLDAVSHLQ WMCEVNRLGS PQYEVHFHHA APDGFLYFAF KVLIPGLPLP LYGFVQILPG TSARAMKSEV YRAAAEQVIQ TLCRVSNLRP F
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
RNA-binding factor that positively regulates gene expression by prohibiting miRNA-mediated gene suppression. Relieves miRNA repression in germline cells. Prohibits the function of several miRNAs by blocking the accessibility of target mRNAs. Sequence-specific RNA-binding factor that binds to U-rich regions (URRs) in the 3'untranslated region (3'-UTR) of several mRNAs. Does not bind to miRNAs. Germline-specific protein required for the primordial germ cell (PGC) survival and migration during early embryonic development.
Gene References into Functions
  1. Homozygous dnd mutants became germ-cell-free males that are able to nurse donor-derived germ cells. PMID: 28731265
  2. study showed that DND protein functions as a translation repressor of specific mRNAs to control PGC development in zebrafish PMID: 28115159
  3. Balance between miRNA-430 function and the action of the RNA-binding protein Dead end, effectively transforms immotile primordial germ cells into polarized cells that actively migrate relative to cells in their environment. PMID: 22705393
  4. Data show that hub RNA is a target of global miRNA-mediated repression, while the RNA-binding protein Dead end (Dnd) contributes to maintenance of the expression in the primordial germ cells (PGCs). PMID: 21337467
  5. identification of target mRNA for ZDnd will be helpful to further explore the biological function of Dnd in zebrafish germ-line development as well as in cancer cells PMID: 20587335
  6. Dnd ATPase is involved in protecting primordial germ cell nanos1 and TDRD7 transcripts. PMID: 20371629
  7. Knockdown of dead end blocks confinement of primordial germ cells (PGCs) to the deep blastoderm shortly after their specification and results in failure of PGCs to exhibit motile behavior and to actively migrate thereafter. PMID: 12932328
  8. Unravel a novel role of dead end 1 (Dnd1) in protecting certain mRNAs from miRNA-mediated repression. PMID: 18155131
  9. Cis-acting elements within dnd mRNA and the RNA recognition motive (RRM) of the protein are essential for targeting protein expression to the germ cells and to the perinuclear granules, respectively. PMID: 19013519

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Subcellular Location
Nucleus. Cytoplasm. Note=Perinuclear germ granules, also called germ plasm or chromatoid body.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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