Recombinant Danio rerio Digestive organ expansion factor (diexf), partial

Code CSB-YP764789DIL
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Source Yeast
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Code CSB-EP764789DIL
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Source E.coli
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Code CSB-EP764789DIL-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP764789DIL
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Source Baculovirus
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Code CSB-MP764789DIL
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
diexf
Uniprot No.
Alternative Names
diexf; defDigestive organ expansion factor
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of the ribosomal small subunit processome for the biogenesis of ribosomes, functions in pre-ribosomal RNA (pre-rRNA) processing. Essential for embryonic development in part through the regulation of p53 pathway. Required for the expansion growth of intestine, liver and exocrine pancreas, but not endocrine pancreas. May not play a major role during the early stage of endodermal organogenesis. Also involved in the sympathetic neuronal development. Triggers the degradation of p53/TP53 and Delta113p53 isoform in a ubiquitination pathway independent way. Mediates, with CAPN3, the proteasome-independent degradation of p53/TP53.
Gene References into Functions
  1. Fibrotic scar formation in def+/- is blocked by the over-expression of Def. PMID: 24801718
  2. Def can mediate the degradation of p53 protein and that this process is independent of the proteasome pathway, but dependent on the activity of Calpain3, a cysteine protease. PMID: 23357851
  3. Def functions in a cell autonomous manner during early liver development. PMID: 23593122
  4. def gene encodes a novel pan-endoderm-specific factor.p53 is tightly regulated by factor Def, especially during organogenesis PMID: 16322560
Subcellular Location
Nucleus, nucleolus.
Protein Families
Def family
Tissue Specificity
Mainly expressed in digestive organs at the later stage of organogenesis. Expressed in liver, gut and exocrine pancreas, but not in the islets.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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