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Recombinant Danio rerio Ephrin-B2a (efnb2a), partial

Recombinant Danio rerio Ephrin-B2a (efnb2a), partial

Code	CSB-YP524228DIL
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP524228DIL
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP524228DIL-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP524228DIL
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP524228DIL
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

efnb2a

Uniprot No.

O73874

Alternative Names

efnb2a; efnb2Ephrin-B2a

Species

Danio rerio (Zebrafish) (Brachydanio rerio)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration.

Gene References into Functions

Notch1 activation induces expression of ephrin b2a (efnb2a) and neuregulin 1 (nrg1) in the endocardium to promote trabeculation and forced Notch activation in the absence of cardiac contraction rescues efnb2a and nrg1 expression PMID: 26628092
We identified ephrinB2a as a target of hif1 pathway activation, showed that knock-down of ephrinB2a rescued the guidance errors, and showed that the receptor ephA4a is expressed in a pattern complementary to the misrouting axons PMID: 22511881
Somite expression of known vascular guidance cues, efnb2, sema3a2, and plexinD1 are disrupted, suggesting that the inter-somitic vessel vascular phenotype is due to disruption of these cues. PMID: 21038448
The ephrinB2a may signal a subpopulation of RGC axons that they have reached their target neurons in the tectum. PMID: 15007827
EphrinB2a is critically involved in increasing the probability of neurons projecting to rhombomere 2 through a reverse signaling mechanism. PMID: 17507550
Data show that EfnB2a is required in developing hindbrain for normal cell affinity and that EphA4 and EfnB2a regulate cell affinity independently within their respective rhombomeres. PMID: 19135438
first embryonic vein formed by selective sprouting of progenitor cells from precursor vessel, followed by vessel segregation; these processes were regulated by EphrinB2 & EphB4 which are expressed in arterial-fated & venous-fated progenitors respectively PMID: 19815777