Recombinant Danio rerio Hepatocyte nuclear factor 1-beta-A (hnf1ba), partial

Code CSB-YP374598DIL
MSDS
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Source Yeast
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Code CSB-EP374598DIL
MSDS
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Source E.coli
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Code CSB-EP374598DIL-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP374598DIL
MSDS
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Source Baculovirus
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Code CSB-MP374598DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
hnf1ba
Uniprot No.
Alternative Names
hnf1ba; hnf1b; tcf2; Hepatocyte nuclear factor 1-beta-A; HNF-1-beta-A; HNF-1B-A; Hepatocyte nuclear factor 1-beta-2; HNF-1B-2; Transcription factor 2; TCF-2; Variant hepatic nuclear factor 1; vHNF1
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor that binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Required for induction of rhombomere r5/r6 gene expression in the hindbrain.
Gene References into Functions
  1. Data show that hnf1ba generates a permissive domain for Wnt signaling activity in the foregut endoderm PMID: 23720049
  2. essential for normal segmentation of nephrons during kidney organogenesis PMID: 23160512
  3. Knockdown of pk1a led to decreased expression of vhnf1, a homeodomain gene previously shown to be involved in biliary development and in kidney cyst formation; forced expression of vhnf1 mRNA led to rescue of the pk1a morphant phenotype PMID: 21215262
  4. Data show that vhnf1 functions in two ways to subdivide the zebrafish caudal hindbrain domain (r4-r7) into individual rhombomeres. PMID: 12835397
  5. Zebrafish tcf2 gene, composed of 9 exons, was mapped to linkage group LG15. PMID: 15302404
  6. vhnf1 acts largely independently of val to repress the rhombomere 4 'hox code' posterior to the rhombomere 4-5 boundary PMID: 15342476
  7. Vhnf1 expression within the posterior neural tube (up to r5) promotes posterior and dorsal fates in the otic vesicle, at the expense of anterior and ventral fates. PMID: 17137573
  8. investigated the signaling pathways that regulate vhnf1 expression during pancreas development PMID: 17217944
  9. Data show that zebrafish mutants for Tcf2 fail to specify a single lumen in their gut tube and instead develop multiple lumens, and show that Tcf2 controls single lumen formation by regulating claudin15 and Na+/K+-ATPase expression. PMID: 17632505
  10. vHNF1 plays crucial role at the earliest steps of liver induction: the acquisition of endoderm competence and the hepatic specification. PMID: 18635606
  11. It was concluded that hnf1b(hi2169) is hypomorphic to hnf1b(hi1843) and that, while hnf1b is required for r5/r6 gene expression in the hindbrain, r5/r6 gene expression can be experimentally induced independently of hnf1b anterior to the hindbrain. PMID: 18945197

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Subcellular Location
Nucleus.
Protein Families
HNF1 homeobox family
Tissue Specificity
During embryonic development, expressed dynamically in the developing hindbrain, kidney (pronephros), gut, liver and pancreas; expressed in both intermediate mesoderm (precursor to the kidney) and the caudal hindbrain (including rhombomeres r5 and r6) at
Database Links
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