Recombinant Danio rerio Homeobox protein orthopedia B (otpb)

Code CSB-YP732227DIL
MSDS
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Source Yeast
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Code CSB-EP732227DIL
MSDS
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Source E.coli
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Code CSB-EP732227DIL-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP732227DIL
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP732227DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
otpb
Uniprot No.
Alternative Names
otpb; otp; otp1; otpa; Homeobox protein orthopedia B
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-315
Target Protein Sequence
MLSHADLLDT RLGMKDAAAE LLVHREALKC RLGGTDSGHP GDLTSATETV EGTTLLPGEE ISNGGSNPNG MQQVNAKDQE KQQQQNSNQT GGQQNQQKQK RHRTRFTPAQ LNELERSFAK THYPDIFMRE ELALRIGLTE SRVQVWFQNR RAKWKKRKKT TNVFRAPGTL LPTHGLPQFP SAAAAAMGDS LCSFHANDTR WAAAGMPGVS QLQIPPSLGR QQAMAQSLSQ CSLGAGPPPN NMGLSGSLSS NGSGLQSHLY QPTFPGMVPA SLSGPTNVTG SPQLCSSPDS DMWRGTSIAS LRRKALEHTV SMSFT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the specification of isotocin cells. Required for the specification of dopaminergic neurons in the developing hypothalamus and posterior tuberculum.
Gene References into Functions
  1. These findings reveal a new role for Otp in controlling developmental neuropeptide balance in a discrete oxytocinergic hypothalamic circuit whose disrupted development affects social behavior. PMID: 28094761
  2. Identification of an evolutionarily conserved vertebrate OTP enhancer module with activity in a restricted area of the forebrain. PMID: 25427861
  3. These observations suggest that the requirement for otpa and otpb function during development of the larval diencephalon is partially redundant. PMID: 24073233
  4. Otp1 transcription factor is important for regulation of diencephalon development and early neuronal progenitors. PMID: 17074092
  5. Orthopedia is one of the few known transcription factors that can determine aspects of the dopaminergic phenotype and the first known factor to control the development of the diencephalospinal dopaminergic system. PMID: 17481897
  6. Results describe the ontogeny of vasotocin-expressing cells in zebrafish, and the selective requirement for the transcriptional regulators orthopedia and single-minded 1 in the preoptic area. PMID: 18330923

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Subcellular Location
Nucleus.
Protein Families
Paired homeobox family, Bicoid subfamily
Tissue Specificity
Isoform 1 and isoform 2 are expressed in brain and testis.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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