Recombinant Danio rerio Pancreas transcription factor 1 subunit alpha (ptf1a)

Code CSB-YP770375DIL
MSDS
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Source Yeast
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Code CSB-EP770375DIL
MSDS
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Source E.coli
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Code CSB-EP770375DIL-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP770375DIL
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP770375DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ptf1a
Uniprot No.
Alternative Names
ptf1a; si:zc142h2.2; zgc:112216; Pancreas transcription factor 1 subunit alpha; Pancreas-specific transcription factor 1a; bHLH transcription factor p48
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-265
Target Protein Sequence
MDTVLDPFTG LDSFSSSYFD DDDFFTDHSS RDHLDTDDFL DEDVDFLTNQ IQEYYKDSRI SQDGDYCDVG NFSFSSSSST FSYGCADSTS ELSPHRDGGL LKRRRRMRSE VEMQQLRQAA NVRERRRMQS INDAFEGLRS HIPTLPYEKR LSKVDTLRLA IGYINFLAEL VQSDMPIRNP HSDALNQPKK VIICHRGTRS PSPNDPDYGL PPLAGHSLSW TDEKQLKDQN IIRTAKVWTP EDPRKLHLKS SINNIENEPP FNFIS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5'-CANNTG-3'. Required for exocrine pancreatic development. Plays a central role in directing the differentiation of retinal progenitors towards horizontal and amacrine fates.
Gene References into Functions
  1. Results highlight the possibility that Ptf1a may act as an important transcription factor for enteric neuron development. PMID: 26865080
  2. In ptf1a haploinsufficiency, more ptf1a-lineage-labeled cells are traced into the pancreatic Notch-responsive cell and endocrine compartments. More reduction of ptf1a gene dosage converts pancreatic progenitor cells to non-pancreatic cell fates. PMID: 25773748
  3. Autoregulation of ptf1a is absolutely required for exocrine pancreas formation. PMID: 23876428
  4. Postembryonic antagonism of Ptf1a, a master regulator of pancreatic development and acinar cell fate specification, induced expression of endocrine genes including insulin in the exocrine compartment. Induced insulin+ cells are derived from acinar cells. PMID: 21497092
  5. The timing of Ptf1a expression suggests that it is involved in the very early stages or steps in the differentiation of amacrine cells, which can be seen to rapidly diversify into a large number of subtypes. PMID: 19732413
  6. mapping, analysis of embryonic expression and role in pancreas development PMID: 15031114
  7. Ptf1 function is inhibited by notch PMID: 15280211
  8. We propose that low levels of Ptf1a promote endocrine fate, whereas high levels repress endocrine fate and promote exocrine fate. PMID: 18519637
  9. Exocrine differentiation and proliferation factor (exdpf) is a direct target gene of pancreas-specific transcription factor 1a (Ptf1a). PMID: 19067490

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Subcellular Location
Nucleus.
Database Links
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